Microbiome and nitrate removal processes by microorganisms on the ancient Preah Vihear temple of Cambodia revealed by metagenomics and N-15 isotope analyses
Microbiome and nitrate removal processes by microorganisms on the ancient Preah Vihear temple of Cambodia revealed by metagenomics and N-15 isotope analyses
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宏基因组学和 N-15 同位素分析揭示了柬埔寨古柏威夏寺微生物组和硝酸盐的去除过程
DOI：
10.1007/s00253-020-10886-4
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发表时间：
2020-09
影响因子：
5
通讯作者：
Gu Ji-Dong
中科院分区：
文献类型：
--
作者：
Ding Xinghua;Lan Wenshen;Wu Jiapeng;Hong Yiguo;Li Yilian;Ge Qingya;Urzì Clara;Katayama Yoko;Gu Ji-Dong
柏威夏寺是被联合国教科文组织列为世界遗产的吴哥古寺庙中最重要的代表之一。这座吴哥砂岩纪念碑的表面覆盖着变质的材料，在这里被广泛称为“沉积物”，这是由于砂岩长期风化的结果。沉积物可能会对这一文化遗产的古砂岩底质产生不利影响，由此产生的潜在风险是当前战略和持续保护和管理的基本信息。从这座寺庙的沉积物样本中提取的DNA用于Illumina的高通量测序分析，产生了大约12 GB的元基因组数据集。这一鸟枪式元基因组分析的结果提供了沉积物微生物组中存在的系统发育群的详细信息，表明该微生物组中可能发生了涉及不同地球微生物旋回的潜在代谢活动。系统发育分析结果表明，大多数元基因组阅读物在门水平上属于变形杆菌、酸性细菌、放线杆菌和拟杆菌。代谢重建结果表明，采样点沉积物中碳的固持和氮硫的转化是重要的地球微生物循环过程。具体来说，异化硝酸盐还原为铵(DNRA)和新发现的完全氨氧化(COAMMOX)是从这个元基因组数据集中检索到的。此外，在这个元基因组数据集中，关于硫和氨氧化细菌和古菌产酸过程的遗传信息表明，这些样品中的微生物区系有可能通过产酸参与砂岩文化遗产的退化。N-15同位素修正和培养分析结果证实了这座寺庙存在活跃的反硝化作用，但没有厌氧氨氧化活性。这些结果对我们了解影响这一砂岩文化遗产的微生物群落组成和微生物生物退化过程具有重要意义，并将有助于古柏威夏寺的保护和管理。利用NGS对柏威夏寺的微生物区系进行分析。·通过DNRA、Comammox和反硝化作用检测到硝酸盐-N的转化。·N-15同位素分析证实了活跃的反硝化作用，但没有证实Anammox。·硝酸盐的积累是反硝化作用去除活性较低的结果。
Preah Vihear temple is one of the most significant representatives of the ancient Angkorian temples listed as United Nations Educational, Scientific and Cultural Organization (UNESCO) World Heritage Sites. The surfaces of this Angkor sandstone monument are covered with deteriorated materials, broadly called “sediments” here, resulting from a long time of weathering of the sandstone. The sediments might adversely affect the ancient sandstone substratum of this cultural heritage, and the potential risk from them is essential information for current strategies and on-going protection and management. The extracted DNA from the sediment samples of this temple was used for Illumina high-throughput sequencing analysis, resulting in approximately 12 Gb of metagenomic dataset. The results of this shotgun metagenomic analysis provided a thorough information of the phylogenetic groups presented in the microbiome of the sediment samples, indicating that potential metabolic activities, involving different geomicrobiological cycles, may occur in this microbiome. The phylogenetic result revealed that the majority of metagenomic reads were affiliated withProteobacteria,Acidobacteria,Actinobacteria, andBacteroidetesat the phylum level. The metabolic reconstruction results indicated that the important geomicrobiological cycling processes include carbon sequestration, and nitrogen and sulfur transformation as the potentially active ones in the sediments of the sampling sites. Specifically, the dissimilatory nitrate reduction to ammonium (DNRA) and the newly discovered complete ammonia oxidation (comammox) were retrieved from this metagenomic dataset. Furthermore, the genetic information on the presence of acid-producing processes by sulfur- and ammonia-oxidizing bacteria and archaea in this metagenomic dataset suggested that the microbial flora in these samples has the potential to participate in the degradation of sandstone cultural heritage by producing acids. N-15 isotope amendment and incubation analysis results confirmed the presence of active denitrification, but not anammox activity at this temple. These results are important for our knowledge on the microbial community composition and microbial biodeterioration processes affecting this sandstone cultural heritage and will aid in the protection and management of the ancient Preah Vihear temple.Key Points•Microbiota on Preah Viher temple was analyzed using NGS.•Nitrate-N transformation by DNRA, comammox, and denitrifcation was detected.•N-15 isotope analysis confirmed the active denitrifcation, but not Anammox.•Accumulation of nitrate is a result of less active removal by denitrification.
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影响因子：
4.4
作者：
M. Strous;J. Kuenen;M. Jetten
通讯作者：
M. Strous;J. Kuenen;M. Jetten
DOI：
10.1186/2042-5783-2-3
发表时间：
2012-02-09
期刊：
Microbial informatics and experimentation
影响因子：
--
作者：
Thomas T;Gilbert J;Meyer F
通讯作者：
Meyer F
影响因子：
5.2
作者：
Kusumi A;Li XS;Katayama Y
通讯作者：
Katayama Y
有氧条件下异养细菌的硫化物产生和氧化
DOI：
10.1038/ismej.2017.125
发表时间：
2017-12
期刊：
The ISME journal
影响因子：
--
作者：
Xia Y;Lü C;Hou N;Xin Y;Liu J;Liu H;Xun L
通讯作者：
Xun L
影响因子：
2.2
作者：
Kusumi A;Li X;Osuga Y;Kawashima A;Gu JD;Nasu M;Katayama Y
通讯作者：
Katayama Y