Tissue flow induces cell shape changes during organogenesis
Tissue flow induces cell shape changes during organogenesis
复制标题
组织流动诱导器官发生过程中细胞形状的变化
DOI：
10.1101/295840
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
M. Manning
中科院分区：
文献类型：
--
作者：
G. Erdemci;Madeline J Clark;Jeffrey D. Amack;M. Manning
在胚胎发育中，细胞形状的变化对于建立功能器官是必不可少的，但在许多情况下，精确调节这些变化的机制仍不清楚。我们认为，器官在周围组织中运动时产生的类似流体的阻力可能会对其结构产生变化，这对其功能是重要的。为了验证这一假设，我们用实验和数学模型研究了斑马鱼的左右组织者--库普弗氏囊泡(KV)。在发育过程中，构成KV的单纤毛细胞沿前后轴经历区域特定的形状变化，这些变化对KV功能至关重要：前细胞变得又长又细，而后细胞变短而蹲下。在这里，我们开发了一个细胞形状的数学顶点模型，它结合了组织流变学和细胞运动学，并使用先前发表的斑马鱼尾芽的流变学数据来约束模型参数[Serwane等人]。以及我们自己对千伏速度的测量。我们发现，KV周围细胞动态产生的阻力足以驱动KV细胞在KV发育过程中的形状变化。更广泛地说，这些结果表明，细胞形状的变化可能是由模型或实验中通常不考虑的动态力驱动的。
In embryonic development, cell shape changes are essential for building functional organs, but in many cases the mechanisms that precisely regulate these changes remain unknown. We propose that fluid-like drag forces generated by the motion of an organ through surrounding tissue could generate changes to its structure that are important for its function. To test this hypothesis, we study the zebrafish left-right organizer, Kupffer’s vesicle (KV), using experiments and mathematical modeling. During development, monociliated cells that comprise the KV undergo region-specific shape changes along the anterior-posterior axis that are critical for KV function: anterior cells become long and thin, while posterior cells become short and squat. Here, we develop a mathematical vertex-like model for cell shapes, which incorporates both tissue rheology and cell motility, and constrain the model parameters using previously published rheological data for the zebrafish tailbud [Serwane et al.] as well as our own measurements of the KV speed. We find that drag forces due to dynamics of cells surrounding the KV could be sufficient to drive KV cell shape changes during KV development. More broadly, these results suggest that cell shape changes could be driven by dynamic forces not typically considered in models or experiments.
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DOI：
10.1073/pnas.0509483102
发表时间：
2005-12-20
影响因子：
11.1
作者：
Gregor, T;Bialek, W;Wieschaus, EF
通讯作者：
Wieschaus, EF
影响因子：
12.5
作者：
Bi, Dapeng;Yang, Xingbo;Manning, M. Lisa
通讯作者：
Manning, M. Lisa
影响因子：
2.7
作者：
Cetera M;Leybova L;Woo FW;Deans M;Devenport D
通讯作者：
Devenport D
影响因子：
4.6
作者：
Kramer-Zucker, AG;Olale, F;Drummond, IA
通讯作者：
Drummond, IA
影响因子：
11.8
作者：
Ewald, Andrew J.;Brenot, Audrey;Werb, Zena
通讯作者：
Werb, Zena