Whole-Genome Transcriptome Analyses of Native Symbionts Reveal Host Coral Genomic Novelties for Establishing Coral-Algae Symbioses.
Whole-Genome Transcriptome Analyses of Native Symbionts Reveal Host Coral Genomic Novelties for Establishing Coral-Algae Symbioses.
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DOI：
10.1093/gbe/evaa240
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发表时间：
2021-01-07
影响因子：
3.3
通讯作者：
Shinzato C
中科院分区：
文献类型：
--
作者：
Yoshioka Y;Yamashita H;Suzuki G;Zayasu Y;Tada I;Kanda M;Satoh N;Shoguchi E;Shinzato C
造礁珊瑚和共生藻科的光合内共生藻类建立了互惠关系，这是珊瑚生物学的基础，使珊瑚礁能够支持各种各样的海洋物种。虽然共生藻科的许多类型出现在珊瑚礁环境中，但鹿角珊瑚在早期生命阶段选择特定的类型。为了研究自然界中发生的珊瑚-藻类共生的分子机制，我们进行了全基因组转录组学分析的细鹿角珊瑚幼虫接种共生藻microadriaticum菌株分离的鹿角珊瑚新兵。为了确定与原生共生体在生命早期阶段的共生具体涉及的基因，我们还研究了鹿角珊瑚幼虫暴露于密切相关的，非共生的，偶尔共生的共生菌菌株的转录组反应。我们发现，差异表达基因的数量是最大的幼虫收购本地共生体。差异表达基因库表明，珊瑚减少了氨基酸，糖和脂质代谢，因此执行这些功能的代谢酶主要来自S. microadriaticum而不是A. tenuis。这些代谢物的转运蛋白的基因表达上调只发生在珊瑚幼虫获得其天然共生体时，这表明宿主珊瑚对原生共生体的积极利用。我们还发现，在鹿角珊瑚中，糖和氨基酸转运蛋白基因，saposin-like和Notch配体样基因，仅在对原生共生体的反应中上调，并且包括串联重复的基因。珊瑚基因组中的基因复制可能是建立珊瑚藻共生的基因组新颖性所必需的。
Reef-building corals and photosynthetic, endosymbiotic algae of the family Symbiodiniaceae establish mutualistic relationships that are fundamental to coral biology, enabling coral reefs to support a vast diversity of marine species. Although numerous types of Symbiodiniaceae occur in coral reef environments, Acropora corals select specific types in early life stages. In order to study molecular mechanisms of coral–algal symbioses occurring in nature, we performed whole-genome transcriptomic analyses of Acropora tenuis larvae inoculated with Symbiodinium microadriaticum strains isolated from an Acropora recruit. In order to identify genes specifically involved in symbioses with native symbionts in early life stages, we also investigated transcriptomic responses of Acropora larvae exposed to closely related, nonsymbiotic, and occasionally symbiotic Symbiodinium strains. We found that the number of differentially expressed genes was largest when larvae acquired native symbionts. Repertoires of differentially expressed genes indicated that corals reduced amino acid, sugar, and lipid metabolism, such that metabolic enzymes performing these functions were derived primarily from S. microadriaticum rather than from A. tenuis. Upregulated gene expression of transporters for those metabolites occurred only when coral larvae acquired their natural symbionts, suggesting active utilization of native symbionts by host corals. We also discovered that in Acropora, genes for sugar and amino acid transporters, prosaposin-like, and Notch ligand-like, were upregulated only in response to native symbionts, and included tandemly duplicated genes. Gene duplications in coral genomes may have been essential to establish genomic novelties for coral–algae symbiosis.
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影响因子：
4.9
作者：
Bertucci, A.;Foret, S.;Miller, D. J.
通讯作者：
Miller, D. J.
影响因子：
4
作者：
Ang, Hwee-Luan;Tergaonkar, Vinay
通讯作者：
Tergaonkar, Vinay
影响因子：
1.6
作者：
Baghdasarian, G;Muscatine, L
通讯作者：
Muscatine, L
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
4.9
作者：
Duncan, Rebecca P.;Husnik, Filip;Wilson, Alex C. C.
通讯作者：
Wilson, Alex C. C.