VqMAPKKK38 is essential for stilbene accumulation in grapevine.
VqMAPKKK38 is essential for stilbene accumulation in grapevine.
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VqMAPKKK38 对于葡萄藤中二苯乙烯的积累至关重要
DOI：
10.1038/hortres.2017.58
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发表时间：
2017
影响因子：
8.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Jiao Y;Wang D;Wang L;Jiang C;Wang Y
包括葡萄藤在内的葡萄属物种产生一类被称为芪类的次级代谢产物，其对植物抗病性很重要，并且可以对人类健康产生积极影响。丝裂原活化蛋白激酶（MAPK）信号通路不仅在植物防御反应中起重要作用，而且参与葡萄藤苯乙烯的生物合成。MAPKKK在MAPK网络的上层发挥作用，并通过该途径启动信号传导。本研究从中国野生葡萄藤（V. quinquangularis）"丹凤2号“中克隆到一个Raf-like MAPKKK基因VqMAPKKK 38，并对其功能进行了分析。我们观察到，VqMAPKKK 38转录水平的白粉病感染，高盐条件和低温胁迫，以及在响应由激素水杨酸（SA），茉莉酸甲酯（MeJA），乙烯（Eth）和脱落酸（阿坝）的处理升高。此外，基于VqMAPKKK 38在葡萄藤叶片中的瞬时过表达和基因抑制，我们发现VqMAPKKK 38可能通过介导转录因子MYB 14的激活来正调控芪合酶的转录和芪的积累。此外，过氧化氢（H2 O2）和钙离子内流均能激活VqMAPKKK 38的表达和芪的生物合成，提示VqMAPKKK 38可能参与了钙离子信号和ROS信号通路。
Vitis species, including grapevine, produce a class of secondary metabolites called stilbenes that are important for plant disease resistance and can have positive effects on human health. Mitogen-activated protein kinase (MAPK) signaling cascades not only play key roles in plant defense responses but also contribute to stilbene biosynthesis in grapevine. MAPKKKs function at the upper level of the MAPK network and initiate signaling through this pathway. In this study, a Raf-like MAPKKK gene, VqMAPKKK38, was identified and functionally characterized from the Chinese wild grapevine V. quinquangularis accession ‘Danfeng-2’. We observed that VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA). In addition, based on both transient overexpression and gene suppression of VqMAPKKK38 in grapevine leaves, we found that VqMAPKKK38 positively regulates stilbene synthase transcription and stilbene accumulation probably by mediating the activation of the transcription factor MYB14. In addition, both hydrogen peroxide (H 2 O 2) and calcium influx activated VqMAPKKK38 expression and stilbene biosynthesis, which suggests that VqMAPKKK38 may be involved in the calcium signaling and ROS signaling pathways.
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影响因子：
5.6
作者：
Çakır B;Kılıçkaya O
通讯作者：
Kılıçkaya O
影响因子：
6.9
作者：
Duan D;Halter D;Baltenweck R;Tisch C;Tröster V;Kortekamp A;Hugueney P;Nick P
通讯作者：
Nick P
影响因子：
6.9
作者：
Jiao Y;Xu W;Duan D;Wang Y;Nick P
通讯作者：
Nick P
影响因子：
6.1
作者：
Gonzalez-Barrio, Rocio;Beltran, David;Tomas-Barberan, Francisco A.
通讯作者：
Tomas-Barberan, Francisco A.
影响因子：
7.2
作者：
Danquah, Agyemang;de Zelicourt, Axel;Colcombet, Jean
通讯作者：
Colcombet, Jean