Development of a biological scaffold engineered using the extracellular matrix secreted by skeletal muscle cells.
Development of a biological scaffold engineered using the extracellular matrix secreted by skeletal muscle cells.
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DOI：
10.1016/j.biomaterials.2015.01.027
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发表时间：
2015-05
期刊：
影响因子：
14
通讯作者：
Wolchok, Jeffrey C.
中科院分区：
文献类型：
--
作者：
Hurd, Shiloh A.;Bhatti, Nadia M.;Walker, Addison M.;Kasukonis, Ben M.;Wolchok, Jeffrey C.
来自脱细胞组织的可植入生物材料的性能，包括骨骼肌的令人鼓舞的结果，表明来自天然组织的细胞外基质（ECM）具有很好的再生潜力。然而，来源于捐赠组织的生物材料的供应总是有限的，这就是为什么模仿组织特性的ECM生物材料的体外制造是一种有吸引力的替代方案。为此，我们的小组利用了一种新的方法来收集骨骼肌成肌细胞分泌的ECM，并将其形成可植入的支架。细胞衍生的ECM含有几种基质成分，包括胶原蛋白和纤连蛋白，其也在骨骼肌样品中鉴定。ECM被组织成多孔网络，该多孔网络可以形成有在肌肉样品内观察到的细长且对齐的结构。ECM材料支持细胞的附着和体外增殖，表明细胞移植的有效性，并且在体内检查时宿主耐受良好。结果表明，ECM收集方法可用于生产具有与肌肉样品相似的组成和结构的生物材料，虽然物理性质可能与肌肉值不匹配，但体外和体内结果表明它可能是组织衍生生物材料的合适的第一代替代品。
The performance of implantable biomaterials derived from decellularized tissue, including encouraging results with skeletal muscle, suggests that the extracellular matrix (ECM) derived from native tissue has promising regenerative potential. Yet, the supply of biomaterials derived from donated tissue will always be limited, which is why the in-vitro fabrication of ECM biomaterials that mimic the properties of tissue is an attractive alternative. Towards this end, our group has utilized a novel method to collect the ECM that skeletal muscle myoblasts secrete and form it into implantable scaffolds. The cell derived ECM contained several matrix constituents, including collagen and fibronectin that were also identified within skeletal muscle samples. The ECM was organized into a porous network that could be formed with the elongated and aligned architecture observed within muscle samples. The ECM material supported the attachment and in-vitro proliferation of cells, suggesting effectiveness for cell transplantation, and was well tolerated by the host when examined in-vivo. The results suggest that the ECM collection approach can be used to produce biomaterials with compositions and structures that are similar to muscle samples, and while the physical properties may not yet match muscle values, the in-vitro and in-vivo results indicate it may be a suitable first generation alternative to tissue derived biomaterials.
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