Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment.
Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment.
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发育中的绵羊乳腺终末导管小叶单位具有动态的粘膜和基质免疫微环境。
DOI：
10.1038/s42003-021-02502-6
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发表时间：
2021-08-20
影响因子：
5.9
通讯作者：
Hughes K
中科院分区：
文献类型：
--
作者：
Nagy D;Gillis CMC;Davies K;Fowden AL;Rees P;Wills JW;Hughes K
人类乳房和绵羊乳腺在出生后发育水平惊人，导致终末导管小叶单位（TDLU）的形成。在这里，我们探讨绵羊 TDLU 生长的各个方面，将其作为乳房发育的模型，并增加对绵羊乳房发育的了解。年轻和年老羔羊上皮细胞核 Ki67 阳性的分布存在显着差异。 Ki67 表达极化至正在发育的 TDLU 的前沿。在接近青春期的羔羊中，上皮内导管巨噬细胞表现出周期性并且密度显着增加。基质巨噬细胞在中央比在外周更丰富。年长羔羊的上皮内 T 淋巴细胞数量较多。 Ki67 表达的基质热点与免疫细胞聚集体共定位，免疫细胞聚集体表现出与三级淋巴结构一致的独特组织。因此，羔羊乳腺表现出动态的粘膜和基质免疫微环境，并构成了一个有价值的模型系统，为产后乳房发育提供了新的见解。纳吉等人。描述了青春期前和围青春期绵羊乳腺发育的组织形态发生，作为人类乳腺发育的候选模型。他们研究了不同年龄的几种免疫细胞类型的位置和丰度，强调这是一个有价值的模型系统，可以为产后乳房发育提供新的见解。
The human breast and ovine mammary gland undergo striking levels of postnatal development, leading to formation of terminal duct lobular units (TDLUs). Here we interrogate aspects of sheep TDLU growth as a model of breast development and to increase understanding of ovine mammogenesis. The distributions of epithelial nuclear Ki67 positivity differ significantly between younger and older lambs. Ki67 expression is polarised to the leading edge of the developing TDLUs. Intraepithelial ductal macrophages exhibit periodicity and considerably increased density in lambs approaching puberty. Stromal macrophages are more abundant centrally than peripherally. Intraepithelial T lymphocytes are more numerous in older lambs. Stromal hotspots of Ki67 expression colocalize with immune cell aggregates that exhibit distinct organisation consistent with tertiary lymphoid structures. The lamb mammary gland thus exhibits a dynamic mucosal and stromal immune microenvironment and constitutes a valuable model system that provides new insights into postnatal breast development. Nagy et al. describe the histomorphogenesis of ovine mammary development in pre- and peri-pubertal sheep as a candidate model for human breast development. They investigate the locale and abundance of several immune cell types at different ages, highlighting this as a valuable model system that can provide new insights into postnatal breast development.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
4.6
作者：
Brady NJ;Chuntova P;Schwertfeger KL
通讯作者：
Schwertfeger KL
影响因子：
3.3
作者：
Akers, R. M.
通讯作者：
Akers, R. M.
影响因子：
5.4
作者：
Hitchcock, Jessica R.;Hughes, Katherine;Watson, Christine J.
通讯作者：
Watson, Christine J.
影响因子：
2.5
作者：
Howard, BA;Gusterson, BA
通讯作者：
Gusterson, BA