Validated single urinary assay designed for exposomic multi-class biomarkers of common environmental exposures.
Validated single urinary assay designed for exposomic multi-class biomarkers of common environmental exposures.
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DOI：
10.1007/s00216-022-04159-4
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发表时间：
2022-08
影响因子：
4.3
通讯作者：
Andra, Syam S.
中科院分区：
文献类型：
--
作者：
Jagani, Ravikumar;Pulivarthi, Divya;Patel, Dhavalkumar;Wright, Rosalind J.;Wright, Robert O.;Arora, Manish;Wolff, Mary S.;Andra, Syam S.
流行病学研究通常需要使用少量生物样本来量化环境化学混合物的痕量暴露的分析方法。目前，尿液中已发现多达150种环境化学物质的极性代谢物。因此，我们开发了一种用于定量尿液中生物标志物的多类方法。在多类方法的方法验证中优化了单次样品制备，然后进行三次LC进样。经验证，该检测方法可量化尿液中的50种暴露生物标志物，属于7个化学类别和16个亚类。这些类别代表了12种个人护理和消费品化学品（PCP），5种多环芳烃（PAH），5种有机磷酸盐阻燃剂（OPFR），18种农药，5种挥发性有机化合物（VOC），4种烟草生物碱和1种滥用药物的代谢物。用同位素标记的内标物加标人尿液（0.2 mL），酶解偶联，固相萃取提取，并使用高效液相色谱-串联质谱法进行分析。将来自净化的甲醇馏分分成两半，并在Betasil C18柱上分析前半部分的PCP、PAH和OPFR;在Hypersil Gold AQ柱上分析农药和VOC。在Synergi Polar RP柱上分析后半部分的烟草烟雾代谢物和滥用药物。尿液的检测限范围为0.01 - 1.0 ng/mL，大多数≤ 0.5 ng/mL（42/50）。分析精度，估计为批内和批间不确定度的相对标准偏差，变异性，<20%。提取回收率范围为83 - 109%。优化的多类方法的结果在正式的国际能力验证项目中合格。进一步的方法定制选项进行了探索和方法扩展被证明包括多达101个分析物内源性和外源性化学品。这种规模庞大的检测方法正用于人口研究，节省了检测成本和生物样本，提供了定量结果和意外暴露的发现。
Epidemiologic studies often call for analytical methods that use a small biospecimen volume to quantify trace level exposures to environmental chemical mixtures. Currently, as many as 150 polar metabolites of environmental chemicals have been found in urine. Therefore, we developed a multi-class method for quantitation of biomarkers in urine. A single sample preparation followed by three LC injections was optimized in a proof-of-approach for a multi-class method. The assay was validated to quantify 50 biomarkers of exposure in urine, belonging to 7 chemical classes and 16 sub-classes. The classes represent metabolites of 12 personal care and consumer product chemicals (PCPs), 5 polycyclic aromatic hydrocarbons (PAH), 5 organophosphate flame retardants (OPFR), 18 pesticides, 5 volatile organic compounds (VOC), 4 tobacco alkaloids, and 1 drug of abuse. Human urine (0.2 mL) was spiked with isotope-labeled internal standards, enzymatically deconjugated, extracted by solid phase extraction, and analyzed using high-performance liquid chromatography-tandem mass spectrometry. The methanol eluate from the cleanup was split in half and the first half analyzed for PCPs, PAH, and OPFR on a Betasil C18 column; and pesticides and VOC on a Hypersil Gold AQ column. The second half was analyzed for tobacco smoke metabolites and a drug of abuse on a Synergi Polar RP column. Limits of detection ranged from 0.01 to 1.0 ng/mL of urine, with the majority ≤ 0.5 ng/mL (42/50). Analytical precision, estimated as relative standard deviation of intra-and inter-batch uncertainty, variabilities, was <20%. Extraction recoveries ranged from 83 to 109%. Results from the optimized multi-class method were qualified in formal international proficiency testing programs. Further method customization options were explored and method expansion was demonstrated by inclusion of up to 101 analytes endo- and exo-genous chemicals. This exposome-scale assay is being used for population studies with savings of assay costs and bio-specimens, providing both quantitative results and discovery of unexpected exposures.
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影响因子：
4.3
作者：
Schantz, Michele M.;Benner, Bruce A., Jr.;Heckert, N. Alan;Sander, Lane C.;Sharpless, Katherine E.;Vander Pol, Stacy S.;Vasquez, Y.;Villegas, M.;Wise, Stephen A.;Alwis, K. Udeni;Blount, Benjamin C.;Calafat, Antonia M.;Li, Zheng;Silva, Manori J.;Ye, Xiaoyun;Gaudreau, Eric;Patterson, Donald G., Jr.;Sjoedin, Andreas
通讯作者：
Sjoedin, Andreas
影响因子：
8.3
作者：
Asimakopoulos, Alexandros G.;Xue, Jingchuan;Kannan, Kurunthachalam
通讯作者：
Kannan, Kurunthachalam
影响因子：
6.2
作者：
Alwis, K. Udeni;Blount, Benjamin C.;Ashley, David L.
通讯作者：
Ashley, David L.
影响因子：
8.8
作者：
Ao, Junjie;Zhang, Qianlong;Zhang, Jun
通讯作者：
Zhang, Jun
DOI：
10.1038/sj.jea.7500322
发表时间：
2004-05-01
期刊：
JOURNAL OF EXPOSURE ANALYSIS AND ENVIRONMENTAL EPIDEMIOLOGY
影响因子：
--
作者：
Bravo, R;Caltabiano, LM;Barr, DB
通讯作者：
Barr, DB