Clonality and distribution of clinical Ureaplasma isolates recovered from male patients and infertile couples in China.
Clonality and distribution of clinical Ureaplasma isolates recovered from male patients and infertile couples in China.
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中国男性患者和不孕夫妇临床分离解脲支原体的克隆和分布
DOI：
10.1371/journal.pone.0183947
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Ruan Z;Yang T;Shi X;Kong Y;Xie X;Zhang J
脲原体属在成人和新生儿患者中作为病原体获得越来越多的认可，具有多种临床表现。然而，在中国的男性人群和不育夫妇中，这种微生物的克隆性在很大程度上是未知的。在这项研究中，96（53 U。parvum和43个U.解脲脲原体（Ureaplasma spp.）采用多位点序列分型（MLST）/扩展多位点序列分型（eMLST）方法对男性患者和15对不育夫妇的生殖道标本中分离的菌株进行了分析。共鉴定出39种序列类型（ST）和53种扩展序列类型（eST），其中3种占优势的ST（ST 1、ST 9和ST 22）和eST（eST 16、eST 41和eST 82）。此外，系统发育分析显示两个不同的集群是高度一致的两个脲原体物种之间的分类差异。男性和女性患者的聚类和亚群分布差异有统计学意义（P < 0.001）。不育夫妇中66.7%的男性和40.0%的女性配偶支原体检测阳性。本研究还获得了很好的一致性的尿液和精液标本之间的尿液和精液标本的脲原体物种的鉴定（k > 0.80）。然而，这种一致性仅在U.不孕夫妇中的解脲支原体。总之，男性和女性患者之间的聚类和亚组的分布存在显著差异。联合解脲支原体更容易在不育夫妇之间传播，并通过特定的流行克隆谱系与临床表现相关。
Ureaplasma spp. have gained increasing recognition as pathogens in both adult and neonatal patients with multiple clinical presentations. However, the clonality of this organism in the male population and infertile couples in China is largely unknown. In this study, 96 (53 U. parvum and 43 U. urealyticum) of 103 Ureaplasma spp. strains recovered from genital specimens from male patients and 15 pairs of infertile couples were analyzed using multilocus sequence typing (MLST)/expanded multilocus sequence typing (eMLST) schemes. A total of 39 sequence types (STs) and 53 expanded sequence types (eSTs) were identified, with three predominant STs (ST1, ST9 and ST22) and eSTs (eST16, eST41 and eST82). Moreover, phylogenetic analysis revealed two distinct clusters that were highly congruent with the taxonomic differences between the two Ureaplasma species. We found significant differences in the distributions of both clusters and sub-groups between the male and female patients (P < 0.001). Moreover, 66.7% and 40.0% of the male and female partners of the infertile couples tested positive for Ureaplasma spp. The present study also attained excellent agreement of the identification of both Ureaplasma species between paired urine and semen specimens from the male partners (k > 0.80). However, this concordance was observed only for the detection of U. urealyticum within the infertile couples. In conclusion, the distributions of the clusters and sub-groups significantly differed between the male and female patients. U. urealyticum is more likely to transmit between infertile couples and be associated with clinical manifestations by the specific epidemic clonal lineages.
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影响因子：
3.7
作者：
Zhang J;Kong Y;Ruan Z;Huang J;Song T;Song J;Jiang Y;Yu Y;Xie X
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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作者：
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