Jumbo Bacteriophages Are Represented Within an Increasing Diversity of Environmental Viruses Infecting the Emerging Phytopathogen, Dickeya solani.
Jumbo Bacteriophages Are Represented Within an Increasing Diversity of Environmental Viruses Infecting the Emerging Phytopathogen, Dickeya solani.
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DOI：
10.3389/fmicb.2018.02169
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发表时间：
2018
影响因子：
5.2
通讯作者：
Salmond GPC
中科院分区：
文献类型：
--
作者：
Day A;Ahn J;Salmond GPC
狄氏菌属是广泛分布于欧洲大陆的重要经济植物病原菌，可使农作物减产25%。没有有效的环境可接受的化学系统可用于由Dickeya引起的疾病。噬菌体已被建议用于在田间对这些病原体进行生物防治，并且已经进行了有限的田间试验。迄今为止，大多数能够感染Dickeya solani（更具侵略性的物种之一）的噬菌体来自同一科，阿克曼病毒科（Ackermannviridae），其中的许多代表已被证明不适合用于该领域，因为它们具有广泛转导的能力。该家族的成员也只能在D.索拉尼在这里，我们描述了新的噬菌体从环境来源分离的D。Solani，包括另外两个病毒家族的成员;肌尾病毒科和短尾病毒科，其中大多数能够在多个Dickeya物种上形成噬斑。全基因组测序显示，Myoviridae家族成员形成了两个新的基因组超过250 kbp的巨型噬菌体簇，其中一个簇含有另一种植物病原体Erwinia amylovora的DNA。转导实验表明，大多数新的环境噬菌体也能够促进有效的水平基因转移，但单一的短尾病毒科家族成员却不能。因此，这种特定的噬菌体有可能用作针对多种Dickeya的生物防治剂。
Dickeya species are economically important phytopathogens widespread in mainland Europe that can reduce crop yields by 25%. There are no effective environmentally-acceptable chemical systems available for diseases caused by Dickeya. Bacteriophages have been suggested for use in biocontrol of these pathogens in the field, and limited field trials have been conducted. To date the majority of bacteriophages capable of infecting Dickeya solani, one of the more aggressive species, are from the same family, the Ackermannviridae, many representatives of which have been shown to be unsuitable for use in the field due to their capacity for generalized transduction. Members of this family are also only capable of forming individual plaques on D. solani. Here we describe novel bacteriophages from environmental sources isolated on D. solani, including members of two other viral families; Myoviridae and Podoviridae, most of which are capable of forming plaques on multiple Dickeya species. Full genomic sequencing revealed that the Myoviridae family members form two novel clusters of jumbo bacteriophages with genomes over 250 kbp, with one cluster containing phages of another phytopathogen Erwinia amylovora. Transduction experiments showed that the majority of the new environmental bacteriophages are also capable of facilitating efficient horizontal gene transfer, however the single Podoviridae family member is not. This particular phage therefore has potential for use as a biocontrol agent against multiple species of Dickeya.
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影响因子：
6.7
作者：
Cianfanelli FR;Alcoforado Diniz J;Guo M;De Cesare V;Trost M;Coulthurst SJ
通讯作者：
Coulthurst SJ
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作者：
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Salmond GPC
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通讯作者：
NGUYEN, TM
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作者：
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通讯作者：
Lavigne, Rob