Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR.
Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR.
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DOI：
10.1186/s13567-021-00935-9
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发表时间：
2021-05-12
影响因子：
4.4
通讯作者：
Aragon V
中科院分区：
文献类型：
--
作者：
Macedo N;Gottschalk M;Strutzberg-Minder K;Van CN;Zhang L;Zou G;Zhou R;Marostica T;Clavijo MJ;Tucker A;Aragon V
通过血清分型PCR、vtaA毒力标记前导序列（LS）-PCR、临床意义和地理区域对副猪Glaesserella parasuis菌株进行了表征。总体而言，血清型4、5/12、7、1和13是最常检出的。最具临床相关性的血清型是全身分离株，其血清型5/12、13或7的概率较高。相比之下，肺部分离株为血清型2、4、7或14的可能性更高。血清型5/12和13以前被认为与疾病相关，但这项研究与最近其他研究一致，表明血清型7确实与全身性G。副猪流感血清型4菌株说明了分离株如何具有不同程度的毒力，并可从肺、全身或鼻部位获得。血清型8、9、15和10主要从鼻样品获得，这表明这些血清型的临床意义有限。此外，大多数内部G。副猪链球菌分离株通过LS-PCR被分类为毒性的，并且是疾病相关分离株，包括血清型1、2、4、5/12、7、13和14。通过LS-PCR将来自鼻腔的分离株（包括血清型6、9、10、11和15）归类为无毒力。因此，G.副猪链球菌血清型保持恒定，很少有血清型代表从受感染猪分离的大多数菌株。此外，还证实了LS-PCR可用于G.全球副猪嗜血杆菌野外菌株毒力预测。在线版本包含补充材料，可通过10.1186/s13567-021-00935-9获得。
Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly detected. Serovars of greatest clinical relevance were systemic isolates that had a higher probability of being serovar 5/12, 13, or 7. In comparison, pulmonary isolates had a higher likelihood of being serovars 2, 4, 7, or 14. Serovars 5/12 and 13 have previously been considered disease-associated, but this study agrees with other recent studies showing that serovar 7 is indeed associated with systemic G. parasuis disease. Serovar 4 strains illustrated how isolates can have varying degrees of virulence and be obtained from pulmonary, systemic, or nasal sites. Serovars 8, 9, 15, and 10 were predominantly obtained from nasal samples, which indicates a limited clinical significance of these serovars. Additionally, most internal G. parasuis isolates were classified as virulent by LS-PCR and were disease-associated isolates, including serovars 1, 2, 4, 5/12, 7, 13, and 14. Isolates from the nasal cavity, including serovars 6, 9, 10, 11, and 15, were classified as non-virulent by LS-PCR. In conclusion, the distribution of G. parasuis serovars remains constant, with few serovars representing most of the strains isolated from affected pigs. Moreover, it was confirmed that the LS-PCR can be used for G. parasuis virulence prediction of field strains worldwide. The online version contains supplementary material available at 10.1186/s13567-021-00935-9.
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影响因子：
4.4
作者：
Costa-Hurtado M;Ballester M;Galofré-Milà N;Darji A;Aragon V
通讯作者：
Aragon V
影响因子：
4.4
作者：
Moleres, Javier;Santos-Lopez, Alfonso;Garmendia, Junkal
通讯作者：
Garmendia, Junkal
影响因子：
2.2
作者：
Palzer, A.;Ritzmann, M.;Heinritzi, K.
通讯作者：
Heinritzi, K.
影响因子：
3.3
作者：
Li, Jiangnan;Wang, Shengnan;Weng, Changjiang
通讯作者：
Weng, Changjiang
影响因子：
3.3
作者：
Angen, O;Svensmark, B;Mittal, KR
通讯作者：
Mittal, KR