Palaeosymbiosis revealed by genomic fossils of Wolbachia in a strongyloidean nematode.
Palaeosymbiosis revealed by genomic fossils of Wolbachia in a strongyloidean nematode.
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DOI：
10.1371/journal.pgen.1004397
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发表时间：
2014-06
期刊：
影响因子：
4.5
通讯作者：
Blaxter M
中科院分区：
文献类型：
--
作者：
Koutsovoulos G;Makepeace B;Tanya VN;Blaxter M
沃尔巴克氏体是陆生节肢动物常见的内共生体，也存在于线虫中：动物寄生丝虫虫和植物寄生丝虫虫。沃尔巴克氏体DNA向宿主基因组的横向转移是常见的。我们生成了一个圆形线虫寄生虫（牛肺虫）的基因组序列草图。在组装中，我们鉴定出近1mb的序列与沃尔巴克氏体相似。这些片段不太可能来自活的沃尔巴克氏体感染：大多数都很短，基因因失活突变而失效。许多片段与明确的线虫衍生序列共同组装。我们发现了有限的沃尔巴克氏体衍生基因表达的证据。研究发现，产弧菌沃尔巴克氏体基因与丝虫菌株和宿主混杂进化枝f的菌株最为相似。我们认为，产弧菌过去曾被沃尔巴克氏体感染过，而f进化枝样共生体可能是丝虫沃尔巴克氏体感染的来源。牛肺虫是经济上重要的牛线虫寄生虫。我们已经对牛肺虫的基因组进行了测序，为药物和疫苗的发现提供了信息。在肺虫基因组中，我们发现了大量证据，证明肺虫和沃尔巴克氏菌之间存在着古老的联系。沃尔巴克氏肺虫现在是基因组中的“化石”，但它讲述了一种古老的感染。以前并不知道肺蠕虫和相关线虫蠕虫与沃尔巴克氏体之间的联系。我们已经使用肺虫沃尔巴克氏体序列来探索线虫-沃尔巴克氏体相互作用的历史，特别是这些共生体在节肢动物和线虫之间的跳跃。
Wolbachia are common endosymbionts of terrestrial arthropods, and are also found in nematodes: the animal-parasitic filaria, and the plant-parasite Radopholus similis. Lateral transfer of Wolbachia DNA to the host genome is common. We generated a draft genome sequence for the strongyloidean nematode parasite Dictyocaulus viviparus, the cattle lungworm. In the assembly, we identified nearly 1 Mb of sequence with similarity to Wolbachia. The fragments were unlikely to derive from a live Wolbachia infection: most were short, and the genes were disabled through inactivating mutations. Many fragments were co-assembled with definitively nematode-derived sequence. We found limited evidence of expression of the Wolbachia-derived genes. The D. viviparus Wolbachia genes were most similar to filarial strains and strains from the host-promiscuous clade F. We conclude that D. viviparus was infected by Wolbachia in the past, and that clade F-like symbionts may have been the source of filarial Wolbachia infections. Bovine lungworms are economically important nematode parasites of cattle. We have sequenced the genome of the bovine lungworm to provide information for drug and vaccine discovery. Within the lungworm genome we found extensive evidence of an ancient association between the lungworm and a bacterium called Wolbachia. The lungworm Wolbachia is now a “fossil” in the genome, but tells of an ancient infection. Association between lungworms, and related nematode worms, and Wolbachia was not known previously. We have used the lungworm Wolbachia sequence to explore the history of nematode-Wolbachia interactions, particularly the jumping of these symbionts between arthropods and nematodes.
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影响因子：
30.8
作者：
Desjardins, Christopher A.;Cerqueira, Gustavo C.;Goldberg, Jonathan M.;Hotopp, Julie C. Dunning;Haas, Brian J.;Zucker, Jeremy;Ribeiro, Jose M. C.;Saif, Sakina;Levin, Joshua Z.;Fan, Lin;Zeng, Qiandong;Russ, Carsten;Wortman, Jennifer R.;Fink, Doran L.;Birren, Bruce W.;Nutman, Thomas B.
通讯作者：
Nutman, Thomas B.
影响因子：
9.6
作者：
Fenn, K;Blaxter, M
通讯作者：
Blaxter, M
影响因子：
4.4
作者：
Ranganathan, Shoba;Nagaraj, Shivashankar H;Hu, Min;Strube, Christina;Schnieder, Thomas;Gasser, Robin B
通讯作者：
Gasser, Robin B
影响因子：
14.9
作者：
Boratyn GM;Camacho C;Cooper PS;Coulouris G;Fong A;Ma N;Madden TL;Matten WT;McGinnis SD;Merezhuk Y;Raytselis Y;Sayers EW;Tao T;Ye J;Zaretskaya I
通讯作者：
Zaretskaya I
影响因子：
16.8
作者：
Fenn, K;Blaxter, M
通讯作者：
Blaxter, M