Transcriptional regulatory network of WOX11 is involved in the control of crown root development, cytokinin signals, and redox in rice.
Transcriptional regulatory network of WOX11 is involved in the control of crown root development, cytokinin signals, and redox in rice.
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WOX11 的转录调控网络参与水稻冠根发育、细胞分裂素信号和氧化还原的控制
DOI：
10.1093/jxb/erx153
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发表时间：
2017-05-17
影响因子：
6.9
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Jiang W;Zhou S;Zhang Q;Song H;Zhou DX;Zhao Y
在水稻冠根发育过程中，WOX11在激素信号、胁迫响应、氧化还原和代谢等多个过程的调控中起着重要的调控作用。水稻的根系主要由胚后茎生根组成，称为冠根。WOX11编码一个与WUSCHEL相关的同源盒域转录因子，是水稻冠根生长发育的关键调控因子。l)。除了特异地激活冠根发育外，WOX11还参与侧根起始、根毛形成和非生物胁迫。然而，WOX11下游的基因调控网络在很大程度上仍然未知。在这里，我们用RNA-Seq方法研究了wox11根尖的转录组，并通过生物信息学和生化分析确定了wOX11的直接结合靶点。转录分析发现了664个差异表达基因，涉及与根发育、细胞分裂素稳态/信号转导、胁迫响应和氧化还原代谢过程相关的广泛功能。生物信息学分析还表明，WOX11结合基序分布在41%(273/664)的差异表达基因中，主要集中在启动子和内含子区域。我们使用qRT-PCR和/或原位杂交来证实一些受WOX11调控的基因在冠根发育中共表达。我们还使用了凝胶迁移率改变分析和染色质免疫沉淀与抗WOX11抗体来验证WOX11对这些基因的直接调控。分析确定了WOX11下游的几个基因，它们在控制冠根形成、细胞分裂素信号、胁迫反应和氧化还原代谢方面起作用。本研究建立了水稻冠根发育过程中WOX11基因的分级调控模型。
Rice WOX11 plays a regulatory role high in the hierarchy of control of multiple processes such as hormone signaling, stress response, redox, and metabolism during crown root development. The rice root system is mainly composed of post-embryonic shoot-borne roots called crown roots. WOX11, encoding a WUSCHEL-related homeobox domain transcription factor, is a key regulator of crown root growth and development in rice (Oryza sativa. L). In addition to specifically activating crown root development, WOX11 is also involved in lateral root initiation, root hair formation, and abiotic stresses. However, the gene regulatory network downstream of WOX11 remains largely unknown. Here, we studied the transcriptome of wox11 root tips by RNA-Seq and determined direct WOX11-binding targets by bioinformatic and biochemical analysis. The transcriptomic analysis revealed 664 differentially expressed genes, which covered a wide range of functions related to root development, cytokinin homeostasis/signaling, stress response, and redox metabolic processes. Bioinformatic analysis also revealed that the WOX11-binding motif was distributed over 41% (273/664) of the differentially expressed genes, and was mostly enriched in the promoter and intron regions. We used qRT-PCR and/or in situ hybridization to confirm co-expression of some of the WOX11-regulated genes in crown root development. We also used electrophoretic mobility shift assay and chromatin immunoprecipitation with anti-WOX11 antibody to validate direct regulation of these genes by WOX11. The analysis identified several genes that acted downstream of WOX11 in controlling crown root formation, cytokinin signaling, stress response, and redox metabolism. This work built a hierarchical regulatory model of WOX11 in rice crown root development.
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影响因子：
9.4
作者：
Baltruschat, Helmut;Fodor, Jozsef;Skoczowski, Andrzej
通讯作者：
Skoczowski, Andrzej
影响因子：
11.6
作者：
Liu, Jingchun;Sheng, Lihong;Xu, Lin
通讯作者：
Xu, Lin
影响因子：
7.4
作者：
Dai, Mingqiu;Hu, Yongfeng;Zhou, Dao-Xiu
通讯作者：
Zhou, Dao-Xiu
影响因子：
4.4
作者：
Coudert Y;Bès M;Le TV;Pré M;Guiderdoni E;Gantet P
通讯作者：
Gantet P
影响因子：
6.9
作者：
Fang Y;Xie K;Xiong L
通讯作者：
Xiong L