Unsymmetrical cyanine dye via in vivo hitchhiking endogenous albumin affords high-performance NIR-II/photoacoustic imaging and photothermal therapy.
Unsymmetrical cyanine dye via in vivo hitchhiking endogenous albumin affords high-performance NIR-II/photoacoustic imaging and photothermal therapy.
复制标题
通过体内搭便车内源性白蛋白的不对称花青染料提供高性能 NIR-II/光声成像和光热治疗
DOI：
10.1186/s12951-021-01075-0
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发表时间：
2021-10-24
影响因子：
10.2
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Xu P;Hu L;Yu C;Yang W;Kang F;Zhang M;Jiang P;Wang J
在此，提出并验证了一种用于开发高性能NIR-II荧光团的前所未有的协同策略。基于不对称花菁染料的设计策略，将对称花菁染料ICG中的吲哚啉供体用一个完全共轭的苯并[c，d]吲哚供体取代，成功地合成了近红外Ⅱ型发射染料NIC。这种微小的结构变化最大限度地保持了花青染料的高消光系数优势。构建了具有内源性白蛋白搭便车能力的NIC-ER，以进一步增强其体内荧光亮度。在HSA（人血清白蛋白）的存在下，NIC-ER自发地驻留在白蛋白口袋中，并且观察到荧光显著增加~89倍。由于其高的摩尔吸光系数和中等的量子产率，NIC-ER在HSA中表现出明亮的NIR-II发射，具有高的光稳定性和显著的斯托克斯位移（>110 nm）。此外，NIC-ER被成功地用于肿瘤靶向NIR-II/PA成像和有效的光热肿瘤消除。总的来说，我们的策略可能会为设计和构建高性能的NIR-II荧光团开辟一条新的途径。在线版本包含补充材料，可通过10.1186/s12951-021-01075-0获得。
Herein, an unprecedented synergistic strategy for the development of high-performance NIR-II fluorophore is proposed and validated. Based on an unsymmetrical cyanine dye design strategy, the NIR-II emissive dye NIC was successfully developed by replacing only one of the indoline donors of symmetrical cyanine dye ICG with a fully conjugated benz[c,d]indole donor. This minor structural change maximally maintains the high extinction coefficient advantage of cyanine dyes. NIC-ER with endogenous albumin-hitchhiking capability was constructed to further enhance its in vivo fluorescence brightness. In the presence of HSA (Human serum albumin), NIC-ER spontaneously resides in the albumin pocket, and a brilliant ~89-fold increase in fluorescence was observed. Due to its high molar absorptivity and moderate quantum yield, NIC-ER in HSA exhibits bright NIR-II emission with high photostability and significant Stokes shift (>110 nm). Moreover, NIC-ER was successfully employed for tumor-targeted NIR-II/PA imaging and efficient photothermal tumor elimination. Overall, our strategy may open up a new avenue for designing and constructing high-performance NIR-II fluorophores. The online version contains supplementary material available at 10.1186/s12951-021-01075-0.
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影响因子：
--
作者：
Liu R;Tang J;Xu Y;Zhou Y;Dai Z
通讯作者：
Dai Z
影响因子：
16.6
作者：
Antaris AL;Chen H;Diao S;Ma Z;Zhang Z;Zhu S;Wang J;Lozano AX;Fan Q;Chew L;Zhu M;Cheng K;Hong X;Dai H;Cheng Z
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Cheng Z
影响因子：
29.4
作者：
Li, Shengliang;Deng, Qingyuan;Lee, Chun-Sing
通讯作者：
Lee, Chun-Sing
影响因子：
16.6
作者：
Naczynski, D. J.;Tan, M. C.;Zevon, M.;Wall, B.;Kohl, J.;Kulesa, A.;Chen, S.;Roth, C. M.;Riman, R. E.;Moghe, P. V.
通讯作者：
Moghe, P. V.
影响因子：
46.2
作者：
Jung HS ;Verwilst P ;Sharma A ;Shin J ;Sessler JL ;Kim JS
通讯作者：
Kim JS