Multiplexed Monitoring of Neurochemicals via Electrografting-Enabled Site-Selective Functionalization of Aptamers on Field-Effect Transistors.
Multiplexed Monitoring of Neurochemicals via Electrografting-Enabled Site-Selective Functionalization of Aptamers on Field-Effect Transistors.
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DOI：
10.1021/acs.analchem.1c05531
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发表时间：
2022-06-21
影响因子：
7.4
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
--
作者：
Gao, Zan;Wu, Guangfu;Song, Yang;Li, Huijie;Zhang, Yuxuan;Schneider, Michael J.;Qiang, Yingqi;Kaszas, Jackson;Weng, Zhengyan;Sun, He;Huey, Bryan D.;Lai, Rebecca Y.;Zhang, Yi
神经化学物质共释放在理解大脑活动和认知方面受到广泛关注。尽管进行了多次尝试，但利用现有方法对协同释放的神经化学物质进行时空精度和最小串扰的多路监测仍然具有挑战性。在这里，我们报道了一种软神经探针，通过电嫁接辅助石墨烯场效应晶体管（g- fet）适配体的位点选择性功能化，用于多路神经化学监测。神经探针具有优异的灵活性，超轻质量（28 mg），每个G-FET的近细胞尺度尺寸为50 μm × 50 μm。作为一个证明，我们表明，具有电化学接枝分子连接体（-COOH或-NH2）和特定适配体的g - fet可以用于监测血清素和多巴胺，具有高灵敏度（检测限：10 pM）和选择性（多巴胺传感器>比去甲肾上腺素高22倍；血清素传感器>比多巴胺高17倍）。此外，我们证明了在磷酸盐缓冲盐水、人工脑脊液和收获的小鼠脑组织中，在单个神经探针中同时监测多巴胺和血清素的可行性，并且具有最小的串扰和干扰。稳定性研究表明，在大鼠脑脊液中培养96小时后，多路神经探针保持了同时监测多巴胺和血清素的能力，并且最小的串扰，尽管在高浓度下观察到传感器响应降低。在收获的小鼠大脑中进行的离体研究表明，在监测多巴胺和血清素的诱发释放方面有潜在的应用。所开发的多路检测方法也可用于监测其他神经化学物质，如氨基酸和神经肽，只需更换g - fet上功能化的适配体。
Neurochemicals corelease has received much attention in understanding brain activity and cognition. Despite many attempts, the multiplexed monitoring of coreleased neurochemicals with spatiotemporal precision and minimal crosstalk using existing methods remains challenging. Here, we report a soft neural probe for multiplexed neurochemical monitoring via electrografting-assisted site-selective functionalization of aptamers on graphene field-effect transistors (G-FETs). The neural probes possess excellent flexibility, ultralight mass (28 mg), and a nearly cellular-scale dimension of 50 μm × 50 μm for each G-FET. As a demonstration, we show that G-FETs with electrochemically grafted molecular linkers (-COOH or -NH2) and specific aptamers can be used to monitor serotonin and dopamine with high sensitivity (limit of detection: 10 pM) and selectivity (dopamine sensor > 22-fold over norepinephrine; serotonin sensor > 17-fold over dopamine). In addition, we demonstrate the feasibility of the simultaneous monitoring of dopamine and serotonin in a single neural probe with minimal crosstalk and interferences in phosphate-buffered saline, artificial cerebrospinal fluid, and harvested mouse brain tissues. The stability studies show that multiplexed neural probes maintain the capability for simultaneously monitoring dopamine and serotonin with minimal crosstalk after incubating in rat cerebrospinal fluid for 96 hours, though a reduced sensor response at high concentrations is observed. Ex vivo studies in harvested mice brains suggest the potential applications in monitoring the evoked release of dopamine and serotonin. The developed multiplexed detection methodology can also be adapted for monitoring other neurochemicals, such as amino acids and neuropeptides, by simply replacing the aptamers functionalized on the G-FETs.
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DOI：
10.1021/acsmeasuresciau.1c00017
发表时间：
2021-12-15
期刊：
ACS measurement science au
影响因子：
--
作者：
Bell SE;Park I;Rubakhin SS;Bashir R;Vlasov Y;Sweedler JV
通讯作者：
Sweedler JV
影响因子：
6.1
作者：
ARTIGAS, F;SARRIAS, MJ;GELPI, E
通讯作者：
GELPI, E
影响因子：
10.8
作者：
Eckmann, Axel;Felten, Alexandre;Casiraghi, Cinzia
通讯作者：
Casiraghi, Cinzia
影响因子：
11
作者：
Joling, Merijn;van den Heuvel, Odile A.;Vriend, Chris
通讯作者：
Vriend, Chris
影响因子：
7.7
作者：
Al-Hasani R;Wong JT;Mabrouk OS;McCall JG;Schmitz GP;Porter-Stransky KA;Aragona BJ;Kennedy RT;Bruchas MR
通讯作者：
Bruchas MR