The effect of cartilage dehydration and rehydration on quantitative ultrashort echo time biomarkers.
The effect of cartilage dehydration and rehydration on quantitative ultrashort echo time biomarkers.
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DOI：
10.21037/qims-23-359
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发表时间：
2023-10-01
影响因子：
2.8
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中科院分区：
文献类型：
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离体软骨样本脱水和天然滑液或生理盐水再水化对定量超短回波时间（UTE）生物标志物的影响尚不清楚。我们的目的是研究软骨脱水-再水化对UTE生物标志物的影响，并比较天然滑液和生理盐水的再水化能力。从接受全膝关节置换术的患者（n=5）中共采集37个软骨样本。将新鲜软骨标本在基线磁共振扫描后暴露于空气中2 h，然后随机分为两组：一组（n=17）浸泡在天然滑液中，另一组（n=20）浸泡在生理盐水中，再水化4 h。评价了新鲜、脱水和再水化软骨样本的基于UTE的生物标志物[T1、绝热T1 r（AdiabT 1 r）、大分子分数（MMF）、磁化传递率（MTR）和T2*]和样本重量。使用新鲜软骨样品的值作为参考标准来评估组间的差异和一致性。在空气中脱水2小时导致显著的重量损失（P=0.000）。T1、AdiabT 1 r、T2* 显著降低，而MMF、MTR显著升高（均P<0.02）。在滑液和生理盐水中再水化后，观察到软骨重量无显著差异，P值分别为0.204和0.769。在滑液中再水化后，T1、AdiabT 1 r、MMF和MTR无显著差异（P>0.0167，Bonferroni校正），而T2*（P=0.001）与新鲜样本相比仍有显著差异。然而，在生理盐水中再水化后，未检测到任何评估的UTE生物标志物的显著差异（均P>0.05）。在新鲜样本和用滑液和生理盐水再水化的样本之间的UTE生物标志物测量的一致性中未检测到差异。碳水化合物脱水导致UTE生物标志物的显著变化。用滑液或生理盐水再水化对所有评价的UTE生物标志物均无显著影响，但T2* 值除外，T2 * 值与用滑液再水化后的新鲜样品相比仍有显著差异。天然滑液和生理盐水的再水化能力无显著差异。
The effect of dehydration of ex vivo cartilage samples and rehydration with native synovial fluid or normal saline on quantitative ultrashort echo time (UTE) biomarkers are unknown. We aimed to investigate the effect of cartilage dehydration-rehydration on UTE biomarkers and to compare the rehydration capabilities of native synovial fluid and normal saline. A total of 37 cartilage samples were harvested from patients (n=5) who underwent total knee replacement. Fresh cartilage samples were exposed to air to dehydrate for 2 hours after baseline magnetic resonance (MR) scanning, then randomly divided into two groups: one soaking in native synovial fluid (n=17) and the other in normal saline (n=20) to rehydrate for 4 hours. UTE-based biomarkers [T1, adiabatic T1r (AdiabT1r), macromolecular fraction (MMF), magnetization transfer ratio (MTR), and T2*] and sample weights were evaluated for fresh, dehydrated, and rehydrated cartilage samples. Differences and agreements between groups were assessed using the values of fresh cartilage samples as reference standard. Dehydrating in air for 2 hours resulted in significant weight loss (P=0.000). T1, AdiabT1r, and T2* decreased significantly while MMF and MTR increased significantly (all P<0.02). Non-significant differences were observed in cartilage weights after rehydrating in both synovial fluid and normal saline, with P values being 0.204 and 0.769, respectively. There were no significant differences in T1, AdiabT1r, MMF, and MTR after rehydrating in synovial fluid (P>0.0167, with Bonferroni correction) while T2* (P=0.001) still had significant differences compared with fresh samples. However, no significant differences were detected for any of the evaluated UTE biomarkers after rehydrating in normal saline (all P>0.05). No differences were detected in the agreement of UTE biomarker measurements between fresh samples and samples rehydrated with synovial fluid and normal saline. Cartilage dehydration resulted in significant changes in UTE biomarkers. Rehydrating with synovial fluid or normal saline had non-significant effect on all the evaluated UTE biomarkers except T2* values, which still had significant differences compared with fresh samples after rehydrating with synovial fluid. No significant difference was observed in the rehydration capabilities of native synovial fluid and normal saline.
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影响因子：
4.4
作者：
Xue, Yan-Ping;Ma, Ya-Jun;Wu, Mei;Jerban, Saeed;Wei, Zhao;Chang, Eric Y.;Du, Jiang
通讯作者：
Du, Jiang
由于微观磁共振成像和生物化学而导致关节软骨的细胞外基质的损害。
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影响因子：
2.8
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影响因子：
4.4
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