Single cell RNA-seq analysis reveals temporally-regulated and quiescence-regulated gene expression in Drosophila larval neuroblasts.
Single cell RNA-seq analysis reveals temporally-regulated and quiescence-regulated gene expression in Drosophila larval neuroblasts.
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DOI：
10.1186/s13064-022-00163-7
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发表时间：
2022-08-24
影响因子：
3.6
通讯作者：
Doe, Chris Q.
中科院分区：
文献类型：
--
作者：
Dillon, Noah;Cocanougher, Ben;Sood, Chhavi;Yuan, Xin;Kohn, Andrea B.;Moroz, Leonid L.;Siegrist, Sarah E.;Zlatic, Marta;Doe, Chris Q.
关键词：
在发育过程中产生神经多样性的机制在很大程度上仍不清楚。在这里，我们使用scRNA-seq方法来发现果蝇幼虫中枢神经系统在几个关键发育时间点的新特征。我们确定了多种祖细胞亚型--包括干细胞样神经母细胞和中间祖细胞--在幼虫发育过程中改变基因表达，并报告了每类祖细胞的新候选标记。我们在新孵化的幼虫中发现了一个静止的神经母细胞池，并表明它们在转录水平上准备好响应胰岛素信号通路退出静止，包括相邻神经胶质信号细胞类型中的相关通路组件。我们确定了候选的“时间转录因子”(TTF)，它们在不同的时间在祖细胞谱系中表达。我们的工作确定了许多细胞类型特定的基因，这些基因是功能角色的候选基因，并对幼虫神经元的分化轨迹产生了新的见解。网上版载有补充材料，可在10.1186/s13064.022-00163-7查阅。
The mechanisms that generate neural diversity during development remains largely unknown. Here, we use scRNA-seq methodology to discover new features of the Drosophila larval CNS across several key developmental timepoints. We identify multiple progenitor subtypes – both stem cell-like neuroblasts and intermediate progenitors – that change gene expression across larval development, and report on new candidate markers for each class of progenitors. We identify a pool of quiescent neuroblasts in newly hatched larvae and show that they are transcriptionally primed to respond to the insulin signaling pathway to exit from quiescence, including relevant pathway components in the adjacent glial signaling cell type. We identify candidate “temporal transcription factors” (TTFs) that are expressed at different times in progenitor lineages. Our work identifies many cell type specific genes that are candidates for functional roles, and generates new insight into the differentiation trajectory of larval neurons. The online version contains supplementary material available at 10.1186/s13064-022-00163-7.
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影响因子：
64.5
作者：
Davie K;Janssens J;Koldere D;De Waegeneer M;Pech U;Kreft Ł;Aibar S;Makhzami S;Christiaens V;Bravo González-Blas C;Poovathingal S;Hulselmans G;Spanier KI;Moerman T;Vanspauwen B;Geurs S;Voet T;Lammertyn J;Thienpont B;Liu S;Konstantinides N;Fiers M;Verstreken P;Aerts S
通讯作者：
Aerts S
DOI：
10.1002/neu.20230
发表时间：
2006-03-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Baqri, R;Charan, R;Ray, K
通讯作者：
Ray, K
影响因子：
10.5
作者：
BIER, E;VAESSIN, H;JAN, YN
通讯作者：
JAN, YN
影响因子：
64.5
作者：
Bainton, RJ;Tsai, LTY;Heberlein, U
通讯作者：
Heberlein, U
影响因子：
64.8
作者：
Bayraktar, Omer Ali;Doe, Chris Q.
通讯作者：
Doe, Chris Q.