Identification of common fungal extracellular membrane (CFEM) proteins in Fusarium sacchari that inhibit plant immunity and contribute to virulence.
Identification of common fungal extracellular membrane (CFEM) proteins in Fusarium sacchari that inhibit plant immunity and contribute to virulence.
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DOI：
10.1128/spectrum.01452-23
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发表时间：
2023-12-12
影响因子：
3.7
通讯作者：
Yao, Wei
中科院分区：
文献类型：
--
作者：
Huang, Zhen;Zhou, Yuming;Li, Huixue;Bao, Yixue;Duan, Zhenzhen;Wang, Caixia;Powell, Charles A.;Wang, Kai;Hu, Qin;Chen, Baoshan;Zhang, Jisen;Zhang, Muqing;Yao, Wei
甘蔗镰刀菌（Fusarium sacchari）是引起我国甘蔗薄皮病的主要病原菌之一。真菌细胞外膜结构域（CFEM）蛋白是真菌特有的一类蛋白，在植物与病原菌的相互作用中发挥重要作用。而F.未鉴定和功能性解析。从F.在本氏烟草（Nicotiana benthamiana）中，克隆了16个FsCFEM基因，并鉴定了4个FsCFEM蛋白（Fs06761、Fs08184、Fs10706和Fs13617）作为抑制BCL 2相关的X蛋白引发的细胞程序性死亡的效应蛋白。亚细胞定位分析表明，这4种效应物均能进入植物细胞，主要定位在细胞膜和细胞核中。通过酵母YTK12分泌系统对4个CFEM蛋白的信号肽进行功能验证。这四种效应蛋白的表达水平在F.受甘蔗感染的甘蔗植物通过真菌基因敲除，发现Fs06761、Fs08184和Fs13617三个基因是病原菌的重要致病因子。我们的研究结果支持FsCFEM效应蛋白是毒力效应子，可能参与调节植物免疫。真菌细胞外膜结构域蛋白（CFEM domain containing protein）一直被认为是一种重要的效应子，在病原菌与植物的互作中起着至关重要的作用。旨在了解F.人们热切期待sacchari最终结束pokkah boeng疾病的传播。在F. sacchari中，并且已经发现四种FsCFEM效应蛋白抑制N.本萨米亚纳这四种效应蛋白具有进入植物细胞并抑制植物免疫的能力。此外，这四种FsCFEM效应蛋白的表达在感染阶段显著增加，其中三种在实现完全毒力方面发挥重要作用。这些研究结果为进一步探索甘蔗的免疫反应提供了方向。通过应用这些发现，我们可以通过宿主诱导的基因沉默等技术来控制疾病的传播。
Fusarium sacchari is one of the primary pathogens causing pokkah boeng disease in sugarcane in China. Common fungal extracellular membrane (CFEM) domain-containing proteins only exist in fungi and play a significant role in the interaction of pathogens and plants. However, the CFEM proteins in F. sacchari were not identified and functionally resolved. Of the 20 FsCFEM proteins identified from the genome of F. sacchari, 16 FsCFEM genes were cloned, while 4 FsCFEM proteins (Fs06761, Fs08184, Fs10706, and Fs13617) were identified as effector proteins that could suppress the programmed cell death triggered by BCL2-associated X protein in Nicotiana benthamiana. Subcellular localization analysis revealed that these four effectors could enter plant cells and are mainly located in the cell membrane and nucleus. By the yeast YTK12 secretion system, the signal peptides of four CFEM proteins were functionally verified. The expression levels of these four effector proteins were significantly increased in the F. sacchari-infected sugarcane plants. Through fungal gene knockout, three genes (Fs06761, Fs08184, and Fs13617) were found to be important pathogenic factors for pathogens. Our results support that the FsCFEM effector proteins were virulence effectors and might be involved in regulating plant immunity. Common fungal extracellular membrane (CFEM) domain-containing protein has long been considered an essential effector, playing a crucial role in the interaction of pathogens and plant. Strategies aimed at understanding the pathogenicity mechanism of F. sacchari are eagerly anticipated to ultimately end the spread of pokkah boeng disease. Twenty FsCFEM proteins in the genome of F. sacchari have been identified, and four FsCFEM effector proteins have been found to suppress BCL2-associated X protein-triggered programmed cell death in N. benthamiana. These four effector proteins have the ability to enter plant cells and inhibit plant immunity. Furthermore, the expression of these four FsCFEM effector proteins significantly increases during the infection stage, with the three of them playing an essential role in achieving full virulence. These study findings provide a direction toward further exploration of the immune response in sugarcane. By applying these discoveries, we can potentially control the spread of disease through techniques such as host-induced gene silencing.
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影响因子：
6.7
作者：
Ai G;Xia Q;Song T;Li T;Zhu H;Peng H;Liu J;Fu X;Zhang M;Jing M;Xia A;Dou D
通讯作者：
Dou D
影响因子：
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作者：
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影响因子：
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作者：
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对坏死性植物病原体的菌核病菌核和辣椒粉预测的分析的种间比较分析。
影响因子：
3.7
作者：
Heard S;Brown NA;Hammond-Kosack K
通讯作者：
Hammond-Kosack K
禾谷镰刀菌 CFEM 蛋白的生物信息学和转录组分析
DOI：
10.3390/jof7100871
发表时间：
2021-10-16
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Chen L;Wang H;Yang J;Yang X;Zhang M;Zhao Z;Fan Y;Wang C;Wang J
通讯作者：
Wang J