Bioinformatics and Transcriptome Analysis of CFEM Proteins in Fusarium graminearum.
Bioinformatics and Transcriptome Analysis of CFEM Proteins in Fusarium graminearum.
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禾谷镰刀菌 CFEM 蛋白的生物信息学和转录组分析
DOI：
10.3390/jof7100871
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发表时间：
2021-10-16
期刊：
影响因子：
--
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Chen L;Wang H;Yang J;Yang X;Zhang M;Zhao Z;Fan Y;Wang C;Wang J
小麦枯萎病通常由禾谷镰刀菌引起，病原菌会向寄主植物组织中分泌效应物质，影响其正常的生理过程，从而使其致病。CFEM蛋白结构域是真菌所特有的，但并非在所有真菌中都有。禾谷镰刀菌中含有的CFEM蛋白可能与致病性密切相关。在本研究中，从禾谷镰刀菌基因组中鉴定了23个FgCFEM蛋白。然后对这些蛋白的信号肽、亚细胞定位、跨膜结构域等特性进行分析，筛选出候选效应子。序列比对结果表明，每个FgCFEM蛋白都含有一个CFEM结构域。CFEM结构域的氨基酸高度保守，含有8个间隔半胱氨酸，但FgCFEM8、9和15缺少两个半胱氨酸，FgCFEM18和FgCFEM22缺少三个半胱氨酸。最近在11个FgCFEM中发现了一个CFEM_DR基序，重要的是，我们在一些FgCFEM蛋白中发现了两个新的保守基序，分别含有约29个和18个氨基酸(CFEM_WR和CFEM_KF)。对CFEM蛋白编码基因的转录组分析表明，在小麦侵染过程中，所有含有CFEM蛋白的基因都得到了表达，其中分别有7个基因和6个基因在植物和试管苗中显著上调和下调。在上述分析的基础上，预测FgCFEM11和FgCFEM23是禾谷镰刀菌的效应子。本研究为进一步研究禾谷镰刀菌CFEM蛋白的功能奠定了基础。
Fusarium blight of wheat is usually caused by Fusarium graminearum, and the pathogenic fungi will secrete effectors into the host plant tissue to affect its normal physiological process, so as to make it pathogenic. The CFEM (Common in Fungal Extracellular Membrane) protein domain is unique to fungi, but it is not found in all fungi. The CFEM protein contained in F. graminearum may be closely related to pathogenicity. In this study, 23 FgCFEM proteins were identified from the F. graminearum genome. Then, features of these proteins, such as signal peptide, subcellular localization, and transmembrane domains, etc., were analyzed and candidate effectors were screened out. Sequence alignment results revealed that each FgCFEM protein contains one CFEM domain. The amino acids of the CFEM domain are highly conserved and contain eight spaced cysteines, with the exception that FgCFEM8, 9, and 15 lack two cysteines and three cysteines were missed in FgCFEM18 and FgCFEM22. A recently identified CFEM_DR motif was detected in 11 FgCFEMs, and importantly we identified two new conserved motifs containing about 29 and 18 amino acids (CFEM_WR and CFEM_KF), respectively, in some of FgCFEM proteins. Transcriptome analysis of the genes encoding CFEM proteins indicated that all the CFEM-containing genes were expressed during wheat infection, with seven and six genes significantly up- and down-regulated, respectively, compared with in planta and in vitro. Based on the above analysis, FgCFEM11 and FgCFEM23 were predicted to be F. graminearum effectors. This study provides the basis for future functional analyses of CFEM proteins in F. graminearum.
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影响因子：
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