Human papillomavirus DNA detection in plasma and cervical samples of women with a recent history of low grade or precancerous cervical dysplasia.
Human papillomavirus DNA detection in plasma and cervical samples of women with a recent history of low grade or precancerous cervical dysplasia.
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DOI：
10.1371/journal.pone.0188592
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Musumeci R
中科院分区：
文献类型：
--
作者：
Cocuzza CE;Martinelli M;Sina F;Piana A;Sotgiu G;Dell'Anna T;Musumeci R
循环HPV DNA先前已在晚期宫颈癌妇女中描述，并被认为是疾病复发和转移的预后标志物。只有少数研究报道了低度或癌前宫颈病变患者血流中存在HPV DNA。本研究的目的是确定是否可以在120名最近有宫颈不典型增生病史的妇女的血浆样本中检测到HPV DNA，这些妇女的病变范围从高度鳞状上皮内病变（H-SIL）到细胞学正常。使用类型特异性实时定量PCR测定法在血浆和宫颈样品中进行HPV DNA检测，鉴定致癌HPV 16、18、31、33、45、51和52。总体而言，34.2%（41/120）的血浆样本显示HPV DNA检测阳性; HPV 45（46.3%）、HPV-51（29.6%）和HPV 16（18.5%）是最常见的基因型。配对的宫颈和血浆样本中HPV检测率随着疾病分期的进展而增加，范围从病变消退的女性的15.4%到HSIL女性的38.9%; HPV 16导致在宫颈和血浆样本中发现HPV DNA阳性的女性中鉴定的最常见基因型。此外，HPV 16在宫颈和血浆样本中均显示出最高的中位病毒载量值，分别为48，313拷贝/104个细胞和1，099拷贝/ml。在这项研究中获得的结果证实，HPV DNA可以检测和定量的无症状宫颈感染的妇女的血浆样本。进一步了解HPV通过宫颈病变妇女血流传播的情况，对于更好地了解HPV感染的自然史及其在其他远处肿瘤中的潜在作用非常重要。
Circulating HPV DNA has been previously described in women with advanced stages of cervical cancer and has been suggested to be a prognostic marker of disease recurrences and metastases. Only a few studies have reported the presence of HPV DNA in bloodstream of patients with low grade or precancerous cervical lesions. This study aimed to define if HPV DNA could be detected in plasma samples of 120 women referred for a recent history of cervical dysplasia who presented with lesions ranging from High Squamous Intraepithelial Lesion (H-SIL) to regressed normal cytology. HPV DNA detection was carried out in both plasma and cervical samples using type-specific real-time quantitative PCR assays identifying oncogenic HPV 16, 18, 31, 33, 45, 51 and 52. Overall, 34.2% (41/120) of plasma samples were shown to be positive for HPV DNA detection; HPV 45 (46.3%), HPV-51 (29.6%), and HPV 16 (18.5%) were the most frequently identified genotypes. The rate of HPV detection in paired cervical and plasma samples increased with advancing disease stage, ranging from 15.4% in women with regressed lesions to 38.9% in women with HSIL; HPV 16 resulted the most common genotype identified in women found to be HPV DNA positive in both cervical and plasma samples. Moreover, HPV 16 showed the highest median viral load value in both cervical and plasma samples, with 48,313 copies/104 cells and 1,099 copies/ml, respectively. Results obtained in this study confirm that HPV DNA can be detected and quantified in plasma samples of women with asymptomatic cervical infection. Further knowledge on HPV dissemination through the blood stream of women with cervical lesions would be very important in better understanding the natural history of HPV infection as well as its potential role in other distant tumors.
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DOI：
10.1007/s00432-016-2251-z
发表时间：
2016-12-01
影响因子：
3.6
作者：
Ilahi, Naureen Ehsan;Anwar, Sobia;Murad, Sheeba
通讯作者：
Murad, Sheeba
影响因子：
1.4
作者：
Rintala, MAM;Grénman, SE;Syrjänen, SM
通讯作者：
Syrjänen, SM
影响因子：
9.4
作者：
Bodaghi, S;Wood, LV;Zheng, ZM
通讯作者：
Zheng, ZM
影响因子：
3.8
作者：
Pornthanakasem W;Shotelersuk K;Termrungruanglert W;Voravud N;Niruthisard S;Mutirangura A
通讯作者：
Mutirangura A
影响因子：
4.8
作者：
Piana AF;Sotgiu G;Muroni MR;Cossu-Rocca P;Castiglia P;De Miglio MR
通讯作者：
De Miglio MR