Esterified trityl radicals as intracellular oxygen probes.
Esterified trityl radicals as intracellular oxygen probes.
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DOI：
10.1016/j.freeradbiomed.2008.12.011
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发表时间：
2009-04-01
影响因子：
7.4
通讯作者：
Zweier, Jay L.
中科院分区：
文献类型：
--
作者：
Liu, Yangping;Villamena, Frederick A.;Sun, Jian;Wang, Tse-Yao;Zweier, Jay L.
关键词：
三芳基甲基（三苯甲基）自由基在生理条件下表现出高稳定性和窄线宽，这为O2浓度的测量提供了高灵敏度和分辨率，使得它们作为EPR血氧测定探针具有吸引力。然而，以前可用的化合物的应用受到其差的细胞内渗透性的限制。我们最近报道了酯化三苯甲基自由基作为潜在的细胞内EPR探针的合成和表征，并研究了它们的氧敏感性，氧化还原性质和酶介导的水解。在本文中，我们报告了这些三苯甲烷在牛主动脉内皮细胞存在下的细胞渗透性和稳定性。结果表明，含乙酰氧基甲氧羰基的三苯甲基AMT-02在细胞存在下表现出高稳定性，并且可以有效地内化。还观察到AMT-02的细胞内水解为三苯甲基（CT-03）的羧酸盐形式。此外，这种内化的三苯甲基探针被应用于测量细胞内O2浓度和甲萘醌和KCN对内皮细胞中O2消耗速率的影响。这项研究表明，这些酯化三苯甲基自由基可以作为有效的EPR血氧探针测量细胞内O2浓度和消耗。
Triarylmethyl (trityl) radicals exhibit high stability and narrow line width at physiological conditions which provide high sensitivity and resolution for the measurement of O2 concentration, making them attractive as EPR oximetry probes. However, the application of previously available compounds has been limited by their poor intracellular permeability. We recently reported the synthesis and characterization of esterified trityl radicals as potential intracellular EPR probes and their oxygen sensitivity, redox properties and enzyme-mediated hydrolysis were investigated. In this paper, we report the cellular permeability and stability of these trityls in the presence of bovine aortic endothelial cells. Results show that the acetoxymethoxycarbonyl-containing trityl AMT-02 exhibits high stability in the presence of cells and can be effectively internalized. The intracellular hydrolysis of AMT-02 to the carboxylate form of the trityl (CT-03) was also observed. In addition, this internalized trityl probe was applied to measure intracellular O2 concentrations and the effects of menadione and KCN on the rates of O2 consumption in endothelial cells. This study demonstrates that these esterified trityl radicals can function as effective EPR oximetry probes measuring intracellular O2 concentration and consumption.
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影响因子：
3.3
作者：
CLARK, LC;MISRAHY, G;FOX, RP
通讯作者：
FOX, RP
影响因子：
2.7
作者：
Dang, Vinh;Wang, Jinhua;Kuppusamy, Periannan
通讯作者：
Kuppusamy, Periannan
影响因子：
5.6
作者：
KJELLSTROM, BT;ORTENWALL, P;RISBERG, B
通讯作者：
RISBERG, B
影响因子：
3.9
作者：
Kutala, VK;Parinandi, NL;Kuppusamy, P
通讯作者：
Kuppusamy, P
DOI：
10.1073/pnas.042671399
发表时间：
2002-02-19
影响因子：
11.1
作者：
Krishna, MC;English, S;Mitchell, JB
通讯作者：
Mitchell, JB