Integration of transcriptomic, proteomic, and metabolomic data to identify lncRNA rPvt1 associations in lipopolysaccharide-treated H9C2 cardiomyocytes.
Integration of transcriptomic, proteomic, and metabolomic data to identify lncRNA rPvt1 associations in lipopolysaccharide-treated H9C2 cardiomyocytes.
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DOI：
10.3389/fgene.2023.1278830
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发表时间：
2023
影响因子：
3.7
通讯作者：
Liu, Chun-Feng
中科院分区：
文献类型：
--
作者：
Zhang, Tie-Ning;Wen, Ri;Yang, Yu-Hang;Yang, Ni;Liu, Chun-Feng
背景：最近的证据表明，长非编码 RNA (lncRNA) rPvt1 在脓毒症心肌组织中升高，并且其敲低可减轻脓毒症引起的心肌损伤。然而，rPvt1 在脓毒症心肌功能障碍中的作用机制尚未阐明。 方法：在本研究中，我们进行了转录组学、蛋白质组学和代谢组学检测，并进行了综合多组学分析，以探讨 rPvt1 与脂多糖 (Lipopoly Sugar) 诱导的 H9C2 心肌细胞损伤之间的关联。 LncRNA rPvt1 沉默是使用慢病毒转导系统实现的。 结果：与阴性对照相比，rPvt1敲低导致转录组、蛋白质组和代谢组发生较大变化。具体而言，通过每个组学分析分别鉴定了 2,385 个差异表达基因（DEG）、272 个差异丰度蛋白质和 75 个差异表达代谢物（DEM）。对这些差异表达/丰富的因子进行基因本体功能注释、京都基因和基因组百科全书、Nr、真核直系同源群和蛋白质直系同源群簇通路分析。结果表明，线粒体能量代谢可能与Pvt1的作用机制密切相关。 结论：这些基因、蛋白质、代谢物及其相关失调途径可能成为研究脓毒症心肌功能障碍中rPvt1调节机制的有前景的靶点，这对于制定预防、诊断和治疗脓毒症心肌损伤的新策略具有重要意义。
Background: Recent evidence has shown that the long non-coding RNA (lncRNA) rPvt1 is elevated in septic myocardial tissues and that its knockdown attenuates sepsis-induced myocardial injury. However, the mechanism underlying the role of rPvt1 in septic myocardial dysfunction has not been elucidated. Methods: In this study, we performed transcriptomic, proteomic, and metabolomic assays and conducted an integrated multi-omics analysis to explore the association between rPvt1 and lipopolysaccharide (Lipopolysaccharide)-induced H9C2 cardiomyocyte injury. LncRNA rPvt1 silencing was achieved using a lentiviral transduction system. Results: Compared to those with the negative control, rPvt1 knockdown led to large changes in the transcriptome, proteome, and metabolome. Specifically, 2,385 differentially expressed genes (DEGs), 272 differentially abundant proteins and 75 differentially expressed metabolites (DEMs) were identified through each omics analysis, respectively. Gene Ontology functional annotation, Kyoto Encyclopedia of Genes and Genomes, Nr, eukaryotic orthologous groups, and Clusters of Orthologous Groups of Proteins pathway analyses were performed on these differentially expressed/abundant factors. The results suggested that mitochondrial energy metabolism might be closely related to the mechanism through which Pvt1 functions. Conclusion: These genes, proteins, metabolites, and their related dysregulated pathways could thus be promising targets for studies investigating the rPvt1-regluatory mechanisms involved in septic myocardial dysfunction, which is important for formulating novel strategies for the prevention, diagnosis and treatment of septic myocardial injury.
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影响因子：
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DOI：
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发表时间：
2022-08-19
期刊：
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影响因子：
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作者：
通讯作者：
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DOI：
10.1007/978-3-319-42059-2_1
发表时间：
2016-01-01
期刊：
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影响因子：
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作者：
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通讯作者：
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