Residual activity of cetrimide and chlorhexidine on Enterococcus faecalis-infected root canals.
Residual activity of cetrimide and chlorhexidine on Enterococcus faecalis-infected root canals.
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DOI：
10.1038/ijos.2013.95
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发表时间：
2014-03
影响因子：
14.9
通讯作者：
Baca, Pilar
中科院分区：
文献类型：
--
作者：
Maria Ferrer-Luque, Carmen;Teresa Arias-Moliz, Maria;Ruiz-Linares, Matilde;Martinez Garcia, Maria Elena;Baca, Pilar
关键词：
有效的终灌方案是根管预备后达到更好的消毒和保证残余抗菌效果的重要步骤。本研究的目的是比较0.2%西甲酰胺、0.2%和2%氯己定在粪肠球菌感染根管内的残留抗菌活性。粪肠杆菌生物膜在单根上培养4周。根管预备后，用17%乙二胺四乙酸（EDTA）冲洗根管，去除涂片层。根根据最终的冲洗液随机分为3个实验组（n=26）：第一组，5 mL 0.2%西曲胺；II组，0.2%氯己定5 mL；第三组，2%氯己定5 mL。样品收集50天，以表明细菌生长的存在。使用非参数Kaplan-Meier生存分析评估50天以上未生长标本的比例。各组间差异采用log-rank检验，P<0.05为差异有统计学意义水平。2%氯己定组的生存率最高，与其他两组相比有统计学差异。50 d时，I组和II组69.23%的标本中检测到粪肠球菌生长，III组为34.61%。0.2%西曲胺与0.2%氯己定之间无显著性差异。在粪肠杆菌感染的根管内，2%氯己定最终灌洗的残留活性高于0.2%氯己定和0.2%西曲胺。
Effective final irrigation regimen is an important step in order to achieve better disinfection and ensure residual antimicrobial effects after root canal preparation. The aim of this study was to compare the residual antimicrobial activity of 0.2% cetrimide, and 0.2% and 2% chlorhexidine in root canals infected with Enterococcus faecalis. Biofilms of E. faecalis were grown on uniradicular roots for 4 weeks. After root canal preparation, root canals were irrigated with 17% ethylenediaminetetraacetic acid (EDTA) to remove the smear layer. The roots were randomly divided into three experimental groups (n=26) according to the final irrigating solution: Group I, 5 mL 0.2% cetrimide; Group II, 5 mL 0.2% chlorhexidine; and Group III, 5 mL 2% chlorhexidine. Samples were collected for 50 days to denote the presence of bacterial growth. The proportion of ungrown specimens over 50 days was evaluated using the nonparametric Kaplan–Meier survival analysis. Differences among groups were tested using the log-rank test and the level of statistical significance was set at P<0.05. The highest survival value was found with 2% chlorhexidine, showing statistically significant differences from the other two groups. At 50 days, E. faecalis growth was detected in 69.23% specimens in Groups I and II, and in 34.61% specimens of Group III. There were no significant differences between 0.2% cetrimide and 0.2% chlorhexidine. Final irrigation with 2% chlorhexidine showed greater residual activity than 0.2% chlorhexidine and 0.2% cetrimide in root canals infected with E. faecalis.
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影响因子：
5
作者：
Gomes, BPFA;Ferraz, CCR;Souza, FJ
通讯作者：
Souza, FJ
影响因子：
4.2
作者：
Portenier, Isabelle;Waltimo, Tuomas;Haapasalo, Markus
通讯作者：
Haapasalo, Markus
影响因子：
4.2
作者：
Baca, Pilar;Luisa Mendoza-Llamas, Maria;Maria Ferrer-Luque, Carmen
通讯作者：
Maria Ferrer-Luque, Carmen
影响因子：
4.2
作者：
Siqueira, JF;Rôças, IN;de Uzeda, M
通讯作者：
de Uzeda, M
影响因子：
4.2
作者：
Distel, JW;Hatton, JF;Gillespie, MJ
通讯作者：
Gillespie, MJ