Revitalization of a diastemal tooth primordium in Spry2 null mice results from increased proliferation and decreased apoptosis.
Revitalization of a diastemal tooth primordium in Spry2 null mice results from increased proliferation and decreased apoptosis.
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DOI：
10.1002/jez.b.21266
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发表时间：
2009-06-15
影响因子：
2.2
通讯作者：
Klein, Ophir D.
中科院分区：
文献类型：
--
作者：
Peterkova, Renata;Churava, Svatava;Lesot, Herve;Rothova, Michaela;Prochazka, Jan;Peterka, Miroslav;Klein, Ophir D.
了解促进或抑制牙齿发育的因素对于设计生物牙齿替代物至关重要。胚胎小鼠牙列是研究这些因素的理想系统，因为它由两种类型的牙齿原基组成。一种类型的原基将继续形成功能性牙齿，而另一种类型的原基启动发育，但在萌芽阶段或之前停止。这种发育停滞有助于无牙小鼠牙间隙的形成。它伴随着未发育的间隙芽的凋亡，这可能是由于抗凋亡信号如成纤维细胞生长因子（FGF）的活性不足。我们以前已经表明，逮捕的一个残牙芽可以拯救失活Spry 2，FGF信号的拮抗剂。在这里，我们研究了上皮细胞死亡和增殖在这一过程中的作用，通过比较Spry 2 −/−和野生型（WT）胚胎下颌骨中发育不全的牙间隙芽（R2）和第一磨牙的发育，使用组织学切片，图像分析和3D重建。与第一磨牙相比，在胚胎第13.5天的WT R2中，发现细胞凋亡显着增加，增殖显着减少。相反，Spry 2 −/−胚胎中FGF信号水平的增加导致R2芽中细胞凋亡的显着减少和增殖的增加。因此，R2参与了额外牙齿原基的形成。对突变小鼠残基牙齿原基再生的研究可以通过增强我们对牙齿形成调控机制的认识，为牙齿再生奠定基础。
An understanding of the factors that promote or inhibit tooth development is essential for designing biological tooth replacements. The embryonic mouse dentition provides an ideal system for studying such factors because it consists of two types of tooth primordia. One type of primordium will go on to form a functional tooth, whereas the other initiates development but arrests at or before the bud stage. This developmental arrest contributes to the formation of the toothless mouse diastema. It is accompanied by the apoptosis of the rudimentary diastemal buds, which presumably results from the insufficient activity of anti-apoptotic signals such as fibroblast growth factors (FGFs). We have previously shown that the arrest of a rudimentary tooth bud can be rescued by inactivating Spry2, an antagonist of FGF signaling. Here, we studied the role of the epithelial cell death and proliferation in this process by comparing the development of a rudimentary diastemal tooth bud (R2) and the first molar in the mandibles of Spry2−/− and wild-type (WT) embryos using histological sections, image analysis and 3D reconstructions. In the WT R2 at embryonic day 13.5, significantly increased apoptosis and decreased proliferation were found compared with the first molar. In contrast, increased levels of FGF signaling in Spry2−/− embryos led to significantly decreased apoptosis and increased proliferation in the R2 bud. Consequently, the R2 was involved in the formation of a supernumerary tooth primordium. Studies of the revitalization of rudimentary tooth primordia in mutant mice can help to lay the foundation for tooth regeneration by enhancing our knowledge of mechanisms that regulate tooth formation.
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影响因子：
3.1
作者：
Kristenova, P;Peterka, M;Peterkova, R
通讯作者：
Peterkova, R
影响因子：
4.6
作者：
Klein, Ophir D.;Lyons, David B.;Balooch, Guive;Marshall, Grayson W.;Basson, M. Albert;Peterka, Miroslav;Boran, Tomas;Peterkova, Renata;Martin, Gail R.
通讯作者：
Martin, Gail R.
影响因子：
56.9
作者：
Kassai, Y;Munne, P;Itoh, N
通讯作者：
Itoh, N
影响因子：
11.8
作者：
Klein, Ophir D.;Minowada, George;Martin, Gail R.
通讯作者：
Martin, Gail R.
影响因子：
7.6
作者：
Boran, T;Lesot, H;Peterkova, R
通讯作者：
Peterkova, R