Genome-wide association study identifies Loci and candidate genes for body composition and meat quality traits in Beijing-You chickens.
Genome-wide association study identifies Loci and candidate genes for body composition and meat quality traits in Beijing-You chickens.
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DOI：
10.1371/journal.pone.0061172
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wen J
中科院分区：
文献类型：
--
作者：
Liu R;Sun Y;Zhao G;Wang F;Wu D;Zheng M;Chen J;Zhang L;Hu Y;Wen J
鸡体成分和肉质性状是鸡的重要经济性状。高通量基因分型平台和相关统计方法的发展使鸡全基因组关联研究成为可能。为了寻找与体成分和肉质性状相关的分子标记和候选基因，利用Illumina 60K SNP微珠芯片对724只北京油鸡进行了全基因组关联研究。每只鸡在100日龄时共测量16个性状，包括屠体重(CW)、全净膛重(EW)、屠宰率、胸肌重(BRW)和胸肌率(BRP)、大腿肌重和百分比、腹脂重量和百分比、胸腿肌干物质和肌内脂肪含量、极限pH值和胸大肌剪切力。利用简单模型(GLM)和压缩混合线性模型(MLM)对与表型性状显著相关的SNP进行了鉴定。在所研究的10个身体成分性状中，有9个已被确定为具有全基因组意义的SNP(P<；2.59E−6)。鸡(Gallus Gallus)第4染色体上的一个一致区域(GGA4)包括7个显著的SNPs和4个候选基因(LCORL、LAP3、LDB2、TAPT1)，与鸡的CW和EW相关。在GGA3上还发现了BRW和BRP的0.65Mb区域。在测量了位于该区域的五个基因在野兽肌肉中的mRNA含量后，发现GJA1表达的变化与胸肌重量在发育过程中的变化是一致的。GJA1基因很可能是鸡胸肌发育的功能基因。在肉质性状方面，确定了几个与肉质性状有关联的SNPs，并讨论了可能的候选基因及其功能。
Body composition and meat quality traits are important economic traits of chickens. The development of high-throughput genotyping platforms and relevant statistical methods have enabled genome-wide association studies in chickens. In order to identify molecular markers and candidate genes associated with body composition and meat quality traits, genome-wide association studies were conducted using the Illumina 60 K SNP Beadchip to genotype 724 Beijing-You chickens. For each bird, a total of 16 traits were measured, including carcass weight (CW), eviscerated weight (EW), dressing percentage, breast muscle weight (BrW) and percentage (BrP), thigh muscle weight and percentage, abdominal fat weight and percentage, dry matter and intramuscular fat contents of breast and thigh muscle, ultimate pH, and shear force of the pectoralis major muscle at 100 d of age. The SNPs that were significantly associated with the phenotypic traits were identified using both simple (GLM) and compressed mixed linear (MLM) models. For nine of ten body composition traits studied, SNPs showing genome wide significance (P<2.59E−6) have been identified. A consistent region on chicken (Gallus gallus) chromosome 4 (GGA4), including seven significant SNPs and four candidate genes (LCORL, LAP3, LDB2, TAPT1), were found to be associated with CW and EW. Another 0.65 Mb region on GGA3 for BrW and BrP was identified. After measuring the mRNA content in beast muscle for five genes located in this region, the changes in GJA1 expression were found to be consistent with that of breast muscle weight across development. It is highly possible that GJA1 is a functional gene for breast muscle development in chickens. For meat quality traits, several SNPs reaching suggestive association were identified and possible candidate genes with their functions were discussed.
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影响因子：
2.4
作者：
Nones, K;Ledur, MC;Coutinho, LL
通讯作者：
Coutinho, LL
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
2.4
作者：
Sahana, G.;Guldbrandtsen, B.;Lund, M. S.
通讯作者：
Lund, M. S.
影响因子：
3.3
作者：
Howell, Gareth R.;Shindo, Mami;Schimenti, John C.
通讯作者：
Schimenti, John C.
DOI：
10.1097/med.0b013e3280122850
发表时间：
2007-02-01
期刊：
Current opinion in endocrinology, diabetes, and obesity
影响因子：
--
作者：
Chandra, Rashmi;Liddle, Rodger A
通讯作者：
Liddle, Rodger A