Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues.
Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues.
复制标题
DOI：
10.1038/s41598-018-25465-x
复制
发表时间：
2018-05-30
影响因子：
4.6
通讯作者：
Stone N
中科院分区：
文献类型：
--
作者：
Ghita A;Matousek P;Stone N
生物组织的光学特性可能受到其温度的影响，从而影响样品内部的光传输。与室温下的实验相比，这可能被利用来在大型生物样品内传递更多的光子，克服由于组织的高度散射性质而造成的一些困难。在这里，我们报告了随着温度从20 ° C升高到40 °C，在生物组织内传输的光的变化，这表明在传输几何结构中探测器收集的光子有相当大的增强。猪组织样品的拉曼信号的测量，大至40 mm厚，表明信号的相当大的增加，从1.3到2倍，受到生物变异性。观察到的增强归因于生物样品中脂质的相变。这表明：1）在室温下对组织进行的实验可能导致低估将在体内体内的身体深度处获得的信号，以及2）可以修改室温下的实验以通过升高感兴趣的材料的温度来增加检测限。
Optical properties of biological tissues can be influenced by their temperature, thus affecting light transport inside the sample. This could potentially be exploited to deliver more photons inside large biological samples, when compared with experiments at room temperature, overcoming some of difficulties due to highly scattering nature of the tissue. Here we report a change in light transmitted inside biological tissue with temperature elevation from 20 to 40 °C, indicating a considerable enhancement of photons collected by the detector in transmission geometry. The measurement of Raman signals in porcine tissue samples, as large as 40 mm in thickness, indicates a considerable increase in signal ranging from 1.3 to 2 fold, subject to biological variability. The enhancements observed are ascribed to phase transitions of lipids in biological samples. This indicates that: 1) experiments performed on tissue at room temperature can lead to an underestimation of signals that would be obtained at depth in the body in vivo and 2) that experiments at room temperature could be modified to increase detection limits by elevating the temperature of the material of interest.
登录
查看更多内容
影响因子：
3.3
作者：
FRAPPART, L;BOUDEULLE, M;FEROLDI, J
通讯作者：
FEROLDI, J
影响因子：
3.3
作者：
Lin, Kan;Wang, Jianfeng;Huang, Zhiwei
通讯作者：
Huang, Zhiwei
影响因子：
3.4
作者：
Lin, Kan;Zheng, Wei;Huang, Zhiwei
通讯作者：
Huang, Zhiwei
参考乳房温度：方程式的建议。
DOI：
10.1590/s1679-45082015ao3392
发表时间：
2015-10
期刊：
Einstein (Sao Paulo, Brazil)
影响因子：
--
作者：
Souza GA;Brioschi ML;Vargas JV;Morais KC;Dalmaso Neto C;Neves EB
通讯作者：
Neves EB
影响因子：
3.5
作者：
Lai, Puxiang;Xu, Xiao;Wang, Lihong V.
通讯作者：
Wang, Lihong V.