Metabolic differences in ripening of Solanum lycopersicum 'Ailsa Craig' and three monogenic mutants.

Metabolic differences in ripening of Solanum lycopersicum 'Ailsa Craig' and three monogenic mutants.
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DOI:
10.1038/sdata.2014.29
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发表时间:
2014
期刊:
影响因子:
9.8
通讯作者:
Steinbeck, Christoph
Steinbeck, Christoph
中科院分区:
综合性期刊2区
文献类型:
--
作者:
Beisken, Stephan;Earll, Mark;Baxter, Charles;Portwood, David;Ament, Zsuzsanna;Kende, Aniko;Hodgman, Charlie;Seymour, Graham;Smith, Rebecca;Fraser, Paul;Seymour, Mark;Salek, Reza M.;Steinbeck, Christoph

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质谱法的应用使得能够检测相关生物体组之间的代谢差异。野生型番茄和三个单基因突变体,成熟抑制剂(rin),非成熟(NOR)和无色非成熟(CNR),番茄的成熟行为方面的代谢指纹的差异被捕获。一个高分辨率的串联质谱系统耦合到液相色谱产生了一个时间序列的成熟行为在离散的时间间隔,重点是花后的变化。使用内标和质量控制来确保系统稳定性。样品和参比化合物的原始数据(包括研究方案)已通过元数据注释工具Isatab保存在开放的代谢组学数据库MetaboLights中,以实现数据集的有效重复使用,例如在代谢组学交叉研究比较或数据融合练习中。
Application of mass spectrometry enables the detection of metabolic differences between groups of related organisms. Differences in the metabolic fingerprints of wild-type Solanum lycopersicum and three monogenic mutants, ripening inhibitor (rin), non-ripening (nor) and Colourless non-ripening (Cnr), of tomato are captured with regard to ripening behaviour. A high-resolution tandem mass spectrometry system coupled to liquid chromatography produced a time series of the ripening behaviour at discrete intervals with a focus on changes post-anthesis. Internal standards and quality controls were used to ensure system stability. The raw data of the samples and reference compounds including study protocols have been deposited in the open metabolomics database MetaboLights via the metadata annotation tool Isatab to enable efficient re-use of the datasets, such as in metabolomics cross-study comparisons or data fusion exercises.
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