Human rheumatoid factor crossidiotypes. I. WA and BLA are heat-labile conformational antigens requiring both heavy and light chains.
Human rheumatoid factor crossidiotypes. I. WA and BLA are heat-labile conformational antigens requiring both heavy and light chains.
复制标题
DOI：
10.1084/jem.164.5.1809
复制
发表时间：
1986-11-01
期刊：
影响因子：
--
通讯作者：
Barnes JL
中科院分区：
文献类型：
--
作者：
Agnello V;Barnes JL
获得的证据表明，WA和BLA交叉体型（XId）组都是需要L和H链的构象抗原，并且随着热变性，定义XId和抗原结合活性的抗原平行丧失。相比之下，一级结构依赖性交叉反应独特型（CRI），PSL 2，这是只有微弱的天然Wa和Bla单克隆类风湿因子（mRF）检测，成为显着的加热Wa和Bla mRF检测。热变性法可能是一种区分构象抗原决定的Ids和一级结构依赖的Ids的简便方法。除热变性外，还发现一些常用于制备RF的酸性条件也引起Id抗原的显著损失。在Bla mRF上发现PSL 2-CRI表明该Id不是WA XId的唯一Id。
Evidence was obtained that both the WA and BLA crossidiotype (XId) groups are conformational antigens requiring both L and H chains and that with heat denaturation the antigens that define the XIds and antigen-binding activity are lost in parallel. In contrast, the primary structure-dependent crossreactive idiotype (CRI), PSL2, which is only weakly detected on native Wa and Bla monoclonal rheumatoid factors (mRFs), became prominently detected on the heated Wa and Bla mRFs. Heat denaturation may provide a simple method for distinguishing Ids determined by conformational antigen from primary structure-dependent Ids. In addition to heat denaturation, some acid conditions commonly used for preparation of RFs were also found to cause marked loss of Id antigen. The finding of PSL2-CRI on Bla mRF indicates that this Id is not unique to the WA XId.
登录
查看更多内容
DOI：
10.1084/jem.139.1.128
发表时间：
1974-01-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kunkel HG;Winchester RJ;Joslin FG;Capra JD
通讯作者：
Capra JD
影响因子：
3.6
作者：
NEWKIRK, M;CHEN, PP;CAPRA, JD
通讯作者：
CAPRA, JD
影响因子：
15.3
作者：
KUNKEL, HG;AGNELLO, V;CAPRA, JD
通讯作者：
CAPRA, JD
影响因子：
15.3
作者：
CHEN, PP;FONG, S;CARSON, DA
通讯作者：
CARSON, DA
影响因子：
15.3
作者：
AGNELLO, V;ARBETTER, A;JOSLIN, F
通讯作者：
JOSLIN, F