Katanin localization requires triplet microtubules in Chlamydomonas reinhardtii.
Katanin localization requires triplet microtubules in Chlamydomonas reinhardtii.
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DOI：
10.1371/journal.pone.0053940
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Dutcher SK
中科院分区：
文献类型：
--
作者：
Esparza JM;O'Toole E;Li L;Giddings TH Jr;Kozak B;Albee AJ;Dutcher SK
中心粒和基体是各种细胞过程所必需的，包括将蛋白质募集到这些结构以实现中心体和纤毛功能。当中心粒/基体组装有缺陷时，这种募集受到损害。引起基底体组装缺陷的突变赋予对紫杉醇的超敏感性。这些包括bld 2、bld 10、bld 12、uni 3、vfl 1、vfl 2和vfl 3。鞭毛运动突变体不赋予敏感性，除了在微管切断蛋白katanin的p60（pf 19）和p80（pf 15）亚基中的突变。我们在筛选紫杉醇敏感性时发现了额外的pf 15和bld 2（ε-微管蛋白）等位基因。衣原体中pf 15和bld 2等位基因是可存活的，不是必需基因。pf 15 -3和bld 2 -6无效等位基因的双突变株的分析表明，在衣原体的基体可能会招募额外的蛋白质以外的katanin影响纺锤体微管的稳定性。bld 2 -5等位基因是一个亚型等位基因，其表型受营养因素的调节。bld 2 -5细胞中的基体缺少近端。基体突变体显示卡他宁的表位标记的p80亚基的异常定位。与IFT蛋白不同，katanin p80不定位于基底体的过渡纤维，这是基于对uni 1突变体的分析以及缺乏katanin p80与IFT 74的共定位。我们认为，三重微管很可能发挥关键作用，而不是过渡纤维所需的IFT本地化的katanin p80招聘的衣原体的基体。
Centrioles and basal bodies are essential for a variety of cellular processes that include the recruitment of proteins to these structures for both centrosomal and ciliary function. This recruitment is compromised when centriole/basal body assembly is defective. Mutations that cause basal body assembly defects confer supersensitivity to Taxol. These include bld2, bld10, bld12, uni3, vfl1, vfl2, and vfl3. Flagellar motility mutants do not confer sensitivity with the exception of mutations in the p60 (pf19) and p80 (pf15) subunits of the microtubule severing protein katanin. We have identified additional pf15 and bld2 (ε-tubulin) alleles in screens for Taxol sensitivity. Null pf15 and bld2 alleles are viable and are not essential genes in Chlamydomonas. Analysis of double mutant strains with the pf15-3 and bld2-6 null alleles suggests that basal bodies in Chlamydomonas may recruit additional proteins beyond katanin that affect spindle microtubule stability. The bld2-5 allele is a hypomorphic allele and its phenotype is modulated by nutritional cues. Basal bodies in bld2-5 cells are missing proximal ends. The basal body mutants show aberrant localization of an epitope-tagged p80 subunit of katanin. Unlike IFT proteins, katanin p80 does not localize to the transition fibers of the basal bodies based on an analysis of the uni1 mutant as well as the lack of colocalization of katanin p80 with IFT74. We suggest that the triplet microtubules are likely to play a key role in katanin p80 recruitment to the basal body of Chlamydomonas rather than the transition fibers that are needed for IFT localization.
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在嗜热四膜虫中，赖氨酸40在α-微管蛋白中的乙酰化并不是必需的。
影响因子：
7.8
作者：
GAERTIG, J;CRUZ, MA;BOWEN, J;GU, L;PENNOCK, DG;GOROVSKY, MA
通讯作者：
GOROVSKY, MA
影响因子：
9.2
作者：
Conduit, Paul T.;Brunk, Kathrin;Raff, Jordan W.
通讯作者：
Raff, Jordan W.
DOI：
10.1083/jcb.98.1.229
发表时间：
1984-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dutcher SK;Huang B;Luck DJ
通讯作者：
Luck DJ
DOI：
10.1083/jcb.141.4.993
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
通讯作者：
Rosenbaum JL
影响因子：
9.2
作者：
Deane, JA;Cole, DG;Rosenbaum, JL
通讯作者：
Rosenbaum, JL