Comparison of articular cartilage repair with different hydrogel-human umbilical cord blood-derived mesenchymal stem cell composites in a rat model.
Comparison of articular cartilage repair with different hydrogel-human umbilical cord blood-derived mesenchymal stem cell composites in a rat model.
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DOI：
10.1186/scrt427
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发表时间：
2014-03-19
影响因子：
7.5
通讯作者：
Park YB
中科院分区：
文献类型：
--
作者：
Chung JY;Song M;Ha CW;Kim JA;Lee CH;Park YB
本研究旨在探讨人脐带血间充质干细胞（hUCB-MSCs）与四种不同水凝胶复合修复大鼠关节软骨的可行性和有效性。在大鼠双侧膝关节股骨转子沟处造成全层关节软骨缺损。hUCB-MSCs与四种不同水凝胶的复合物（A组，4%透明质酸; B组，3%藻酸盐：30%普朗尼克（1：1，v/v）; C组，4%透明质酸：3%藻酸盐：20%普朗尼克（2：1：1，v/v）;和D组，4%透明质酸：3%藻酸盐：20%普朗尼克;壳聚糖（4：1：1：2，v/v）。然后移植到每个研究组（5只大鼠/组）的右膝缺损中。左膝关节移植相应的水凝胶，不含hUCB-MSC作为对照。在移植后16周，软骨修复程度进行了评估宏观和组织学上使用Masson的三色，番红-O，天狼星红染色，和II型胶原免疫染色。总的来说，4%透明质酸水凝胶A组在大体和组织学上导致上级软骨修复，并实现了模仿相邻未损伤关节软骨的细胞排列和胶原组织模式。免疫组化和番红-O染色也显示A组显示最大面积的II型胶原染色。天狼星红染色显示A组胶原纤维束的组织形态更接近正常软骨。本研究的结果表明，人脐血间充质干细胞可用于修复关节软骨缺损在体内和透明质酸是一个有吸引力的水凝胶候选人与人脐血间充质干细胞组合使用。
The present work was designed to explore the feasibility and efficacy of articular cartilage repair using composites of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) and four different hydrogels in a rat model. Full-thickness articular cartilage defects were created at the trochlear groove of femur in both knees of rats. Composites of hUCB-MSCs and four different hydrogels (group A, 4% hyaluronic acid; group B, 3% alginate:30% pluronic (1:1, v/v); group C, 4% hyaluronic acid: 3% alginate: 20% pluronic (2:1:1, v/v}; and group D, 4% hyaluronic acid:3% alginate:20% pluronic;chitosan (4:1:1:2, v/v).) were then transplanted into right knee defect in each study group (five rats/group). Left knees were transplanted with corresponding hydrogels without hUCB-MSCs as controls. At 16 weeks post-transplantation, degrees of cartilage repair were evaluated macroscopically and histologically using Masson’s Trichrome, safranin-O, Sirius red staining, and type-II collagen immunostaining. Overall, group A with 4% hyaluronic acid hydrogel resulted in superior cartilage repair grossly and histologically and achieved a cellular arrangement and collagen organization pattern mimicking adjacent uninjured articular cartilage. Immunostaining and safranin-O staining also revealed that group A displayed the largest areas of type II collagen staining. Sirius red staining revealed that the organization pattern of collagen bundles was more similar to normal cartilage in group A. No evidence of rejection was found. The results of this study suggest that hUCB-MSCs could be used to repair articular cartilage defects in vivo and that hyaluronic acid is an attractive hydrogel candidate for use in combination with hUCB-MSCs.
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影响因子：
4.2
作者：
Chung, Dai-Jung;Choi, Chi-Bong;Kim, Hwi-Yool
通讯作者：
Kim, Hwi-Yool
影响因子：
4.9
作者：
Chung, Cindy;Mesa, John;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
4
作者：
Caplan, Arnold I.;Dennis, James E.
通讯作者：
Dennis, James E.
影响因子：
2.8
作者：
Chaipinyo, K;Oakes, BW;Van Damme, MPI
通讯作者：
Van Damme, MPI
影响因子：
1.9
作者：
Hao, Lei;Zhang, Cheng;Wang, Qing-yu
通讯作者：
Wang, Qing-yu