The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants.
The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants.
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DOI：
10.1016/j.biomaterials.2010.08.073
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发表时间：
2011-01
期刊：
影响因子：
14
通讯作者：
Lawson, Jeffrey H.
中科院分区：
文献类型：
--
作者：
Achneck, Hardean E.;Jamiolkowski, Ryan M.;Jantzen, Alexandra E.;Haseltine, Justin M.;Lane, Whitney O.;Huang, Jessica K.;Galinat, Lauren J.;Serpe, Michael J.;Lin, Fu-Hsiung;Li, Madison;Parikh, Amar;Ma, Liqiao;Chen, Tao;Sileshi, Bantayehu;Milano, Carmelo A.;Wallace, Charles S.;Stabler, Thomas V.;Allen, Jason D.;Truskey, George A.;Lawson, Jeffrey H.
植入式和体外心血管装置通常由钛 (Ti) 制成（例如镀钛镍钛合金支架和机械循环辅助装置）。这些装置的血液接触钛表面内皮化将为它们提供模仿血管和心脏天然内壁的抗血栓形成涂层。我们评估了外周血来源的猪内皮祖细胞 (EPC) 的活力和粘附性，这些细胞在静态条件下和暴露于流体剪切应力后接种到载玻片上的薄钛层上。 EPC 在无血清培养基中附着并生长至 Ti 上，无需预吸附蛋白质。附着在 Ti 上 15 分钟后，不到 5% 的细胞在 100 达因/cm2 的剪切应力下脱落。光滑 Ti 表面上汇合的 EPC 单层（Rq 为 10 nm），暴露于 15 或 100 dyne/cm2 下 48 小时，沿流动方向排列和伸长，并根据剪切应力水平产生一氧化氮。与未涂层的钛表面相比，EPC 涂层的钛表面显着降低了血小板粘附力。这些结果表明，外周血来源的 EPC 在 Ti 表面粘附并正常发挥功能。因此，EPC 可用于在植入之前植入心血管装置，以改善血小板活化和血栓形成。
Implantable and extracorporeal cardiovascular devices are commonly made from titanium (Ti) (e.g. Ti-coated Nitinol stents and mechanical circulatory assist devices). Endothelializing the blood-contacting Ti surfaces of these devices would provide them with an antithrombogenic coating that mimics the native lining of blood vessels and the heart. We evaluated the viability and adherence of peripheral blood-derived porcine endothelial progenitor cells (EPCs), seeded onto thin Ti layers on glass slides under static conditions and after exposure to fluid shear stresses. EPCs attached and grew to confluence on Ti in serum-free medium, without preadsorption of proteins. After attachment to Ti for 15 min, less than 5 % of the cells detached at a shear stress of 100 dyne/cm2. Confluent monolayers of EPCs on smooth Ti surfaces (Rq of 10 nm), exposed to 15 or 100 dyne/cm2 for 48 hours, aligned and elongated in the direction of flow and produced nitric oxide dependent on the level of shear stress. EPC-coated Ti surfaces had dramatically reduced platelet adhesion when compared to uncoated Ti surfaces. These results indicate that peripheral blood-derived EPCs adhere and function normally on Ti surfaces. Therefore EPCs may be used to seed cardiovascular devices prior to implantation to ameliorate platelet activation and thrombus formation.
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