Phenotypic analyses, protein localization, and bacteriostatic activity of Drosophila melanogaster transferrin-1.
Phenotypic analyses, protein localization, and bacteriostatic activity of Drosophila melanogaster transferrin-1.
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DOI：
10.1016/j.ibmb.2022.103811
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发表时间：
2022-08
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
--
作者：
转铁蛋白-1（Transferrin-1，Tsf 1）是一种对铁有高度亲和力的昆虫胞外蛋白。Tsf 1的功能仍然知之甚少;然而，果蝇Tsf 1已被证明影响苍蝇体内的铁分布，并保护苍蝇免受某些感染。本研究的目的是更好地了解Tsf 1在D.通过1）调查Tsf 1无效表型，2）确定Tsf 1的组织特异性定位，3）测量Tsf 1在血淋巴中的浓度，4）测试Tsf 1的抑菌活性，和5）评估金属和百草枯处理对Tsf 1丰度的影响，来确定黑腹果蝇的Tsf 1基因。缺乏Tsf 1的果蝇在专门的中肠细胞中比野生型果蝇有更多的铁，这些细胞从饮食中吸收铁;然而，缺乏Tsf 1对整个中肠，脂肪体，血淋巴或头部的铁含量没有影响。因此，正如以前的研究表明，Tsf 1似乎在铁转运中起次要作用。Tsf 1在幼虫（0.4 μM）、蛹（1.4 μM）、雌性成虫（4.4 μM）和雄性成虫（22 μM）的血淋巴中含量丰富。Apo-Tsf 1在1 μM时具有抑菌活性，而holo-Tsf 1没有，这表明Tsf 1可以通过螯合血淋巴和其他细胞外环境中的铁来抑制微生物生长。这一假设得到了支持分泌的Tsf 1在气管，睾丸和精囊检测。Tsf 1与卵母细胞中的内体标记物共定位表明Tsf 1可能为发育中的卵子提供铁;然而，缺乏Tsf 1的母亲的卵子与对照卵子具有相同量的铁，并且它们以野生型速率孵化。因此，卵母细胞摄取Tsf 1的主要功能可能是防御感染，而不是为卵子提供铁。在甲虫中，Tsf 1在保护免受氧化应激方面发挥作用。相反，我们发现缺乏Tsf 1的果蝇具有典型的寿命和对百草枯诱导的氧化应激的更大抵抗力。此外，Tsf 1丰度保持不变，在铁，镉或百草枯的摄入或铁注射。这些结果表明，Tsf 1在保护D.黑腹菌
Transferrin-1 (Tsf1) is an extracellular insect protein with a high affinity for iron. The functions of Tsf1 are still poorly understood; however, Drosophila melanogaster Tsf1 has been shown to influence iron distribution in the fly body and to protect flies against some infections. The goal of this study was to better understand the physiological functions of Tsf1 in D. melanogaster by 1) investigating Tsf1 null phenotypes, 2) determining tissue-specific localization of Tsf1, 3) measuring the concentration of Tsf1 in hemolymph, 4) testing Tsf1 for bacteriostatic activity, and 5) evaluating the effect of metal and paraquat treatments on Tsf1 abundance. Flies lacking Tsf1 had more iron than wild-type flies in specialized midgut cells that take up iron from the diet; however, the absence of Tsf1 had no effect on the iron content of whole midguts, fat body, hemolymph, or heads. Thus, as previous studies have suggested, Tsf1 appears to have a minor role in iron transport. Tsf1 was abundant in hemolymph from larvae (0.4 μM), pupae (1.4 μM), adult females (4.4 μM) and adult males (22 μM). Apo-Tsf1 at 1 μM had bacteriostatic activity whereas holo-Tsf1 did not, suggesting that Tsf1 can inhibit microbial growth by sequestering iron in hemolymph and other extracellular environments. This hypothesis was supported by detection of secreted Tsf1 in tracheae, testes and seminal vesicles. Colocalization of Tsf1 with an endosome marker in oocytes suggested that Tsf1 may provide iron to developing eggs; however, eggs from mothers lacking Tsf1 had the same amount of iron as control eggs, and they hatched at a wild-type rate. Thus, the primary function of Tsf1 uptake by oocytes may be to defend against infection rather than to provide eggs with iron. In beetles, Tsf1 plays a role in protection against oxidative stress. In contrast, we found that flies lacking Tsf1 had a typical life span and greater resistance to paraquat-induced oxidative stress. In addition, Tsf1 abundance remained unchanged in response to ingestion of iron, cadmium or paraquat or to injection of iron. These results suggest that Tsf1 has a limited role in protection against oxidative stress in D. melanogaster.
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影响因子：
5.6
作者：
Calap-Quintana P;González-Fernández J;Sebastiá-Ortega N;Llorens JV;Moltó MD
通讯作者：
Moltó MD
影响因子：
3.8
作者：
Brummett LM;Kanost MR;Gorman MJ
通讯作者：
Gorman MJ
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
7
作者：
Casas-Vila N;Bluhm A;Sayols S;Dinges N;Dejung M;Altenhein T;Kappei D;Altenhein B;Roignant JY;Butter F
通讯作者：
Butter F
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE