Distribution of basic fibroblast growth factor in the 18-day rat fetus: localization in the basement membranes of diverse tissues.
Distribution of basic fibroblast growth factor in the 18-day rat fetus: localization in the basement membranes of diverse tissues.
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DOI：
10.1083/jcb.110.3.753
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发表时间：
1990-03
期刊：
影响因子：
--
通讯作者：
Baird A
中科院分区：
文献类型：
--
作者：
Gonzalez AM;Buscaglia M;Ong M;Baird A
采用免疫组化方法研究碱性FGF在18天大鼠胎儿中的分布。结果揭示了一种广泛而特异的染色模式，与碱性 FGF 的广泛分布一致。免疫反应性碱性 FGF 与间充质结构、中胚层和神经外胚层衍生细胞及其细胞外基质有关。例如，骨骼肌细胞和平滑肌细胞呈强阳性。上皮细胞下方的基底膜始终含有碱性 FGF。在某些组织（即软骨和骨）中，免疫染色的强度取决于细胞分化的阶段。尽管组织的染色主要与细胞外基质相关，但各种细胞类型中都存在显着的细胞内染色。这在肾上腺皮质、睾丸和卵巢的内分泌细胞中尤其明显。本文报道的组织化学结果支持这样的观点，即碱性 FGF 具有介导间充质对细胞生长和分化的许多影响所需的特性。讨论了这些发现对于理解碱性 FGF 在调节细胞增殖和分化中的作用的重要性。
Immunohistochemical methods were used to study the distribution of basic FGF in the 18-d rat fetus. The results reveal a pattern of widespread yet specific staining that is consistent with the wide distribution of basic FGF. Immunoreactive basic FGF is associated with mesenchymal structures, mesoderm- and neuroectoderm-derived cells, and their extracellular matrices. As an example, skeletal and smooth muscle cells are strongly positive. The basement membrane underlying the epithelia always contain basic FGF. In some tissues (i.e., cartilage and bone) the intensity of immunostaining is dependent on the stage of cell differentiation. Although the staining of tissues is primarily associated with the extracellular matrix, there is significant intracellular staining in various cell types. This is particularly evident in the endocrine cells of the adrenal cortex, testis, and ovary. The histochemical findings reported here support the notion that basic FGF has the characteristics required to mediate many of the effects of the mesenchyme on cell growth and differentiation. The significance of these findings in understanding the role of basic FGF in regulating cell proliferation and differentiation is discussed.
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