The role of substratum compliance of hydrogels on vascular endothelial cell behavior.
The role of substratum compliance of hydrogels on vascular endothelial cell behavior.
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DOI：
10.1016/j.biomaterials.2011.03.054
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发表时间：
2011-08
期刊：
影响因子：
14
通讯作者：
Murphy, Christopher J.
中科院分区：
文献类型：
--
作者：
Wood, Joshua A.;Shah, Nihar M.;McKee, Clayton T.;Hughbanks, Marissa L.;Liliensiek, Sara J.;Russell, Paul;Murphy, Christopher J.
心血管疾病（CVD）仍然是美国（US）和全球范围内的主要死亡原因。仅在2006年，美国就有超过三分之一的死亡归因于CVD。CVD的高患病率、死亡率、发病率和社会经济影响促使了大量的研究工作;然而，关于疾病的发作和进展以及对改进治疗方法的迫切需求仍然存在显著的知识差距。受到有限关注的一个关键研究领域是生物物理学线索对内皮细胞行为调节的作用;特别是局部顺应性或周围血管内皮细胞外基质硬度的影响。在这项研究中，基底顺应性对几种人原代内皮细胞类型的细胞行为的调制的影响，代表不同的解剖部位和体内分化状态，进行了研究。我们研究中使用的基质涵盖了已报告的血管内皮基底膜的顺应性范围。基质顺应性的差异对细胞附着、铺展、伸长、增殖和迁移产生了深远的影响。此外，每个细胞群体的差异反应的变化，基底顺应性，记录内皮异质性的生物物理线索的反应。这些结果表明，在体外实验的设计，以及未来的假体设计纳入基层顺应性的重要性。血管基质顺应性的改变直接影响内皮细胞行为，并可能参与CVD的发作和/或进展。
Cardiovascular disease (CVD) remains a leading cause of death both within the United States (US) as well as globally. In 2006 alone, over one-third of all deaths in the US were attributable to CVD. The high prevalence, mortality, morbidity, and socioeconomic impact of CVD has motivated a significant research effort; however, there remain significant knowledge gaps regarding disease onset and progression as well as pressing needs for improved therapeutic approaches. One critical area of research that has received limited attention is the role of biophysical cues on the modulation of endothelial cell behaviors; specifically, the impact of local compliance, or the stiffness, of the surrounding vascular endothelial extracellular matrix. In this study, the impact of substratum compliance on the modulation of cell behaviors of several human primary endothelial cell types, representing different anatomic sites and differentiation states in vivo, were investigated. Substrates used within our studies span the range of compliance that has been reported for the vascular endothelial basement membrane. Differences in substratum compliance had a profound impact on cell attachment, spreading, elongation, proliferation, and migration. In addition, each cell population responded differentially to changes in substratum compliance, documenting endothelial heterogeneity in the response to biophysical cues. These results demonstrate the importance of incorporating substratum compliance in the design of in vitro experiments as well as future prosthetic design. Alterations in vascular substratum compliance directly influence endothelial cell behavior and may participate in the onset and/or progression of CVDs.
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影响因子：
4.9
作者：
Jacot, Jeffrey G.;Dianis, Scott;Wong, Joyce Y.
通讯作者：
Wong, Joyce Y.
影响因子：
1.9
作者：
Engler, AJ;Richert, L;Discher, DE
通讯作者：
Discher, DE
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE
影响因子：
8.2
作者：
Klein, R.;Knudtson, M. D.;Klein, B. E. K.;Zinman, B.;Gardiner, R.;Suissa, S.;Sinaiko, A. R.;Donnelly, S. M.;Goodyer, P.;Strand, T.;Mauer, M.
通讯作者：
Mauer, M.
影响因子：
4.9
作者：
Liliensiek, S. J.;Campbell, S.;Murphy, C. J.
通讯作者：
Murphy, C. J.