Dietary administration of scallion extract effectively inhibits colorectal tumor growth: cellular and molecular mechanisms in mice.
Dietary administration of scallion extract effectively inhibits colorectal tumor growth: cellular and molecular mechanisms in mice.
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DOI：
10.1371/journal.pone.0044658
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yang NS
中科院分区：
文献类型：
--
作者：
Arulselvan P;Wen CC;Lan CW;Chen YH;Wei WC;Yang NS
结直肠癌是一种常见的恶性肿瘤，也是全世界癌症死亡的主要原因。众所周知，饮食在结肠癌的病因学中发挥着重要作用，并且膳食化学预防在结肠癌的预防和/或替代治疗方面正受到越来越多的关注。 Allium fistulosum L.，俗称大葱，在世界范围内广泛用作香料或蔬菜，并且在亚洲文化中作为治疗多种疾病的传统药物。在这项研究中，我们使用结肠癌小鼠模型（CT-26 细胞皮下接种到 BALB/c 小鼠中）评估了膳食大葱对结肠癌化学预防的可能有益作用。对肿瘤裂解物进行蛋白质印迹分析以分析关键炎症标志物，进行ELISA以分析细胞因子，并进行免疫组织化学分析以分析炎症标志物。通过 LC-MS/MS 分析大葱提取物的代谢谱。大葱提取物，特别是热水提取物，以50毫克（干重）/公斤体重口服给小鼠，可显着抑制肿瘤生长，并提高试验小鼠的存活率。在分子水平上，与载体对照治疗相比，大葱提取物抑制关键炎症标志物 COX-2 和 iNOS，并抑制已知参与肿瘤凋亡（凋亡指数）、增殖（细胞周期蛋白 D1 和 c-Myc）、血管生成（VEGF 和 HIF-1α）和肿瘤侵袭（MMP-9 和 ICAM-1）的各种细胞标志物的表达 老鼠。我们的研究结果可能需要进一步研究使用普通大葱作为化学预防饮食剂来降低结肠癌的风险。
Colorectal cancer is a common malignancy and a leading cause of cancer death worldwide. Diet is known to play an important role in the etiology of colon cancer and dietary chemoprevention is receiving increasing attention for prevention and/or alternative treatment of colon cancers. Allium fistulosum L., commonly known as scallion, is popularly used as a spice or vegetable worldwide, and as a traditional medicine in Asian cultures for treating a variety of diseases. In this study we evaluated the possible beneficial effects of dietary scallion on chemoprevention of colon cancer using a mouse model of colon carcinoma (CT-26 cells subcutaneously inoculated into BALB/c mice). Tumor lysates were subjected to western blotting for analysis of key inflammatory markers, ELISA for analysis of cytokines, and immunohistochemistry for analysis of inflammatory markers. Metabolite profiles of scallion extracts were analyzed by LC-MS/MS. Scallion extracts, particularly hot-water extract, orally fed to mice at 50 mg (dry weight)/kg body weight resulted in significant suppression of tumor growth and enhanced the survival rate of test mice. At the molecular level, scallion extracts inhibited the key inflammatory markers COX-2 and iNOS, and suppressed the expression of various cellular markers known to be involved in tumor apoptosis (apoptosis index), proliferation (cyclin D1 and c-Myc), angiogenesis (VEGF and HIF-1α), and tumor invasion (MMP-9 and ICAM-1) when compared with vehicle control-treated mice. Our findings may warrant further investigation of the use of common scallion as a chemopreventive dietary agent to lower the risk of colon cancer.
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影响因子：
3
作者：
Chen, JH;Tsai, SJ;Chen, HI
通讯作者：
Chen, HI
影响因子：
3.8
作者：
Lee, Sel-Jung;Lim, Kye-Taek
通讯作者：
Lim, Kye-Taek
DOI：
10.1111/j.1440-1746.2007.05181.x
发表时间：
2008-01-01
影响因子：
4.1
作者：
Lee, Sun-Il;Moon, Hong-Young;Kim, Seon-Han
通讯作者：
Kim, Seon-Han
影响因子：
11.5
作者：
Lin, Yi-Chu;Shun, Chia-Tung;Chen, Ching-Chow
通讯作者：
Chen, Ching-Chow
影响因子：
7.5
作者：
Dymicka-Piekarska, V.;Kemona, H.
通讯作者：
Kemona, H.