Quantification of choline- and ethanolamine-containing metabolites in human prostate tissues using 1H HR-MAS total correlation spectroscopy.
Quantification of choline- and ethanolamine-containing metabolites in human prostate tissues using 1H HR-MAS total correlation spectroscopy.
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DOI：
10.1002/mrm.21647
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发表时间：
2008-07
影响因子：
3.3
通讯作者：
Kurhanewicz, John
中科院分区：
文献类型：
--
作者：
Swanson, Mark G.;Keshari, Kayvan R.;Tabatabai, Z. Laura;Simko, Jeffry P.;Shinohara, Katsuto;Carroll, Peter R.;Zektzer, Andrew S.;Kurhanewicz, John
一种快速和定量的二维高分辨率魔角旋转（HR-MAS）总相关光谱（TOCSY）实验，以解决和定量的胆碱和乙醇胺的代谢产物在人前列腺组织中的病理分析前101小时。在40 ms的混合时间，磁化传递效率常数经验确定在溶液中，并用于计算组织中的代谢物浓度。11/15例（73%）癌组织中观察到磷酸胆碱（PC），但仅6/32例（19%）良性组织。前列腺癌组织中PC显著较高（0.39 ± 0.40 mmol/kg vs. 0.02 ± 0.07 mmol/kg，z = 3.5），而乙醇胺（Eth）显著较低（1.0 ± 0.8 mmol/kg vs. 2.3 ± 1.9 mmol/kg，z = 3.3）。甘油磷酸胆碱（GPC）（0.57 ± 0.87 mmol/kg vs. 0.29 ± 0.26 mmol/kg，z = 1.2），磷酸乙醇胺（PE）（4.4 ± 2.2 mmol/kg vs 3.4 ± 2.6 mmol/kg，z = 1.4）和甘油磷酸乙醇胺（GPE）（0.54 ± 0.82 mmol/kg vs. 0.15 ± 0.15 mmol/kg，z = 1.8）在癌症中高于良性前列腺组织。PC/GPC比值（3.5 ± 4.5 vs. 0.32 ± 1.4，z = 2.6），PC/PE（0.08 ± 0.08 vs. 0.01 ± 0.03，z = 3.5），PE/Eth在癌组织中GPE/Eth（16 ± 22 vs. 2.2 ± 2.0，z = 2.4）和GPE/Eth（0.41 ± 0.51 vs. 0.06 ± 0.06，z = 2.6）也显著高于良性组织。所有样品在HR-MAS分析后均可进行病理学解释;然而，降解实验表明，在1 ° C和2250 Hz旋转速率下1小时内，PC、GPC、PE和GPE降低7.7 ± 2.2%，而Cho + mI和Eth增加18%。
A fast and quantitative 2D high-resolution magic angle spinning (HR-MAS) total correlation spectroscopy (TOCSY) experiment was developed to resolve and quantify the choline- and ethanolamine-containing metabolites in human prostate tissues in ≈1 hr prior to pathologic analysis. At a 40-ms mixing time, magnetization transfer efficiency constants were empirically determined in solution and used to calculate metabolite concentrations in tissue. Phosphocholine (PC) was observed in 11/15 (73%) cancer tissues but only 6/32 (19%) benign tissues. PC was significantly higher (0.39 ± 0.40 mmol/kg vs. 0.02 ± 0.07 mmol/kg, z = 3.5), while ethanolamine (Eth) was significantly lower in cancer versus benign prostate tissues (1.0 ± 0.8 mmol/kg vs. 2.3 ± 1.9 mmol/kg, z = 3.3). Glycerophosphocholine (GPC) (0.57± 0.87 mmol/kg vs. 0.29± 0.26 mmol/kg, z = 1.2), phosphoethanolamine (PE) (4.4± 2.2 mmol/kg vs. 3.4± 2.6 mmol/kg, z = 1.4), and glycerophosphoethanolamine (GPE) (0.54± 0.82 mmol/kg vs. 0.15± 0.15 mmol/kg, z = 1.8) were higher in cancer versus benign prostate tissues. The ratios of PC/GPC (3.5± 4.5 vs. 0.32± 1.4, z = 2.6), PC/PE (0.08± 0.08 vs. 0.01± 0.03, z = 3.5), PE/Eth (16± 22 vs. 2.2± 2.0, z = 2.4), and GPE/Eth (0.41± 0.51 vs. 0.06± 0.06, z = 2.6) were also significantly higher in cancer versus benign tissues. All samples were pathologically interpretable following HR-MAS analysis; however, degradation experiments showed that PC, GPC, PE, and GPE decreased 7.7± 2.2%, while Cho+mI and Eth increased 18% in 1 hr at 1°C and a 2250 Hz spin rate.
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影响因子：
2.9
作者：
KURHANEWICZ, J;DAHIYA, R;NARAYAN, P
通讯作者：
NARAYAN, P
影响因子：
2.9
作者：
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2.2
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2.9
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2.9
作者：
Martínez-Bisbal, MC;Martí-Bonmatí, L;Celda, B
通讯作者：
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