The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant.
The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant.
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硫化氢供体 NaHS 通过作为抗氧化剂延迟大麦糊粉层中的程序性细胞死亡
DOI：
10.1155/2015/714756
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发表时间：
2015
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Zhang YX;Hu KD;Lv K;Li YH;Hu LY;Zhang XQ;Ruan L;Liu YS;Zhang H
H2S是植物和动物中的信号分子。本文研究了H2S对大麦细胞程序性死亡（PCD）的影响。糊粉层H2S供体NaHS显着延迟PCD糊粉层从吸胀的无胚大麦籽粒。0.25 mM NaHS有效降低了糊粉层中超氧阴离子（·O2-）、过氧化氢（H2 O2）和丙二醛（MDA）的积累，提高了超氧化物歧化酶（SOD）、愈创木酚过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的活性，降低了脂氧合酶（LOX）的活性。定量PCR表明，NaHS处理糊粉层组织导致增强的抗氧化基因HvSOD 1，HvAPX，HvCAT 1和HvCAT 2的转录水平和抑制的HvLOX（脂氧合酶基因）和两个半胱氨酸蛋白酶基因HvEPA和HvCP 3 -31的转录水平。赤霉素（GA）处理糊粉层中NaHS处理也延迟了PCD过程，降低了·O2-含量，提高了POD活性，降低了LOX活性。此外，无论GA的存在与否，NaHS处理均能促进大麦糊粉层α-淀粉酶的分泌。这些数据表明，H2S作为一种抗氧化剂，在延迟PCD和促进α-淀粉酶分泌的大麦糊粉层中，无论是否存在GA。
H2S is a signaling molecule in plants and animals. Here we investigated the effects of H2S on programmed cell death (PCD) in barley (Hordeum vulgare L.) aleurone layers. The H2S donor NaHS significantly delayed PCD in aleurone layers isolated from imbibed embryoless barley grain. NaHS at 0.25 mM effectively reduced the accumulation of superoxide anion (·O2 −), hydrogen peroxide (H2O2), and malondialdehyde (MDA), promoted the activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and decreased those of lipoxygenase (LOX) in isolated aleurone layers. Quantitative-PCR showed that NaHS treatment of aleurone tissue led to enhanced transcript levels of the antioxidant genes HvSOD1, HvAPX, HvCAT1, and HvCAT2 and repressed transcript levels of HvLOX (lipoxygenase gene) and of two cysteine protease genes HvEPA and HvCP3-31. NaHS treatment in gibberellic acid- (GA-) treated aleurone layers also delayed the PCD process, reduced the content of ·O2 −, and increased POD activity while decreasing LOX activity. Furthermore, α-amylase secretion in barley aleurone layers was enhanced by NaHS treatment regardless of the presence or absence of GA. These data imply that H2S acted as an antioxidant in delaying PCD and enhances α-amylase secretion regardless of the presence of GA in barley aleurone layers.
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影响因子：
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