The endocytic activity of dendritic cells.
The endocytic activity of dendritic cells.
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DOI：
10.1084/jem.182.2.283
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发表时间：
1995-08-01
影响因子：
15.3
通讯作者：
SWANSON, J
中科院分区：
文献类型：
--
作者：
STEINMAN, RM;SWANSON, J
在这一期中，Sallusto等人(1)报道了树突状细胞。可以通过大吞噬作用大力内化溶质，将这些溶质输送到富含MHC II类的囊泡(MIIV)。在最常见的内吞形式中，包被网状蛋白的凹陷形成小的(直径0.1/~m)包被的残留物(图1)。配体吸附在包被的凹坑中的受体上，随后被输送到溶酶体，在溶酶体中，配体被降解到氨基酸水平。在发生在膜褶皱部位的巨噬细胞吞噬过程中，较大的液滴进入细胞，似乎需要基于肌动蛋白的细胞骨架，而不是网状蛋白(图1)。Sallusto等人(1)描述了无论是在液相中还是在膜上附着的溶质都能迅速进入大小管体内的树突状细胞。在这两种情况下，溶质都被输送到MIIV，在那里它们可能被转化为多肽，并作为MHC II-多肽复合体循环到细胞表面。这一系列事件将解释这些作者之前关于树突状细胞呈递蛋白质抗原的效率的报告(2)。在这项研究中，树突状细胞对抗原的呈递，显然是从液体相，与通过表面Ig受体吸附相同抗原的B细胞系的呈递一样有效。这些新的观察结果代表了事件的惊人转折。在大多数以前对树突状细胞的研究中，很少或没有摄取
I n this issue, Sallusto et al.(1) report that dendritic cells. can vigorously internalize solutes by macropinocytosis, delivering these to MHC class II-rich vesicles (MIIVs). In the most familiar form of endocytosis, clathrin-coated pits invaginate to form small (< 0.1/~ m in diameter) coated resides (Fig. 1). Ligands adsorb to receptors in the coated pit and are subsequently delivered to lysosomes, where the ligands are degraded down to the level of amino acids. During macropinocytosis, which occurs at sites of membrane ruffling, larger droplets of fluid enter the cell, and an actin-based cytoskeleton rather than clathrin seems to be required (Fig. 1). Sallusto et al.(1) describe solutes that rapidly enter dendritic cells within macropinosomes, both in the fluid phase or attached to the membrane. In both cases, the solutes are delivered to MIIVs, where they presumably can be converted to peptides and recycled to the cell surface as MHC II-peptide complexes. This set of events would explain a previous report from these same authors on the efficiency with which dendritic cells present protein antigens (2). In that study, the presentation by dendritic cells of antigens, apparently from the fluid phase, was as effective as presentation by B cell lines that adsorb the same antigen via surface Ig receptors. These new observations represent a striking turn of events. In most previous studies of dendritic cells, little or no uptake
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影响因子：
56.9
作者：
HUNT, DF;MICHEL, H;SETTE, A
通讯作者：
SETTE, A
影响因子：
--
作者：
KELLY, RH;BALFOUR, BM;GRIFFITHS, S
通讯作者：
GRIFFITHS, S
影响因子：
15.3
作者：
Crowley, M;Inaba, K;Steinman, R M
通讯作者：
Steinman, R M
DOI：
10.1083/jcb.83.1.82
发表时间：
1979-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Haigler HT;McKanna JA;Cohen S
通讯作者：
Cohen S
DOI：
10.1083/jcb.124.5.689
发表时间：
1994-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hewlett LJ;Prescott AR;Watts C
通讯作者：
Watts C