An acetylcholinesterase antibody-based quartz crystal microbalance for the rapid identification of spinal ventral and dorsal roots.
An acetylcholinesterase antibody-based quartz crystal microbalance for the rapid identification of spinal ventral and dorsal roots.
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基于乙酰胆碱酯酶抗体的石英晶体微天平，用于快速识别脊髓腹根和背根。
DOI：
10.1371/journal.pone.0069049
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cao X
中科院分区：
文献类型：
--
作者：
Sui T;Ge Y;Liu W;Zhao ZK;Zhang N;Cao X
脊神经前根和脊神经后根乙酰胆碱酯酶（AChE）水平的差异可用于区分脊神经。虽然许多方法可用于测定乙酰胆碱酯酶，一个快速和灵敏的方法还没有以前开发。在这里，我们描述了一种基于抗体的石英晶体微天平（QCM）测定和其应用程序的腹侧和背侧脊神经根的解决方案中的乙酰胆碱酯酶的定量。QCM设备的频率变化对应于AChE在宽动态范围（0.5-10 µg/ml）内的水平，这与ELISA方法的响应范围相当。由前根引起的频率偏移是由背根引起的频率偏移的3倍。基于抗体的QCM传感器在许多连续的重复样品中是稳定的，并且该方法需要不到10分钟，包括AChE提取和分析步骤。该方法是一种快速、简便的生物样品AChE定量方法，可用于外科手术中区分前、后根。
Differences in the levels of acetylcholinesterase (AChE) in ventral and dorsal spinal roots can be used to differentiate the spinal nerves. Although many methods are available to assay AChE, a rapid and sensitive method has not been previously developed. Here, we describe an antibody-based quartz crystal microbalance (QCM) assay and its application for the quantification of AChE in the solutions of ventral and dorsal spinal roots. The frequency variation of the QCM device corresponds to the level of AChE over a wide dynamic range (0.5–10 µg/ml), which is comparable to the response range of the ELISA method. The frequency shift caused by the ventral roots is 3-fold greater than that caused by the dorsal roots. The antibody-based QCM sensor was stable across many successive replicate samples, and the method required less than 10 min, including the AChE extraction and analysis steps. This method is a rapid and convenient means for the quantification of AChE in biological samples and may be applicable for distinguishing the ventral and dorsal roots during surgical operations.
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