Unbiased Stereological Estimates of Dopaminergic and GABAergic Neurons in the A10, A9, and A8 Subregions in the Young Male Macaque.
Unbiased Stereological Estimates of Dopaminergic and GABAergic Neurons in the A10, A9, and A8 Subregions in the Young Male Macaque.
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DOI：
10.1016/j.neuroscience.2022.06.018
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发表时间：
2022-08-01
期刊：
影响因子：
3.3
通讯作者：
Fudge, Julie L.
中科院分区：
文献类型：
--
作者：
Kelly, Emily A.;Contreras, Jancy;Duan, Annie;Vassell, Rochelle;Fudge, Julie L.
关键词：
腹侧中脑是大多数物种中多巴胺（DA）表达神经元的主要来源。GABA能和多巴胺能细胞群混杂在DA表达细胞中，据称可调节局部和远程多巴胺神经元活性。大多数工作已经在啮齿动物模型中进行，但是由于灵长类动物腹侧中脑的进化扩张，DA亚群的大小和复杂性增加，需要进一步研究。在这里，我们定量了猕猴中经典DA细胞群A10（中线腹侧被盖区核[VTA]和臂旁色素核[PBP]）、A9（黑质，丘脑部[SNc]）和A8（红核后区[RRF]）中DA神经元及其GABA能补体的数量。由于PBP是A10组的不成比例的扩展特征，并且在猴子中具有独特的连接特征，因此我们通过将其分为“经典”中线核和PBP来分析A10数据。无偏体视学显示，DA神经元总数为210，238 +/− 17，127（A10 = 110，319 +/− 9，649，A9= 87，399 +/− 7，751和A8= 12，520 +/− 827）。推测的GABA能神经元总体上较少，并且均匀地分布在DA亚群中（GAD 67 = 71，215 +/− 5，663; A10= 16，836 +/− 2，743; A9= 24，855 +/− 3，144和A8= 12，633 +/− 3，557）。计算GAD 67/TH比为每个子区域显示这两种细胞类型的差异平衡的DA亚区。与TH阳性神经元相比，A8亚区具有最高的GAD 67阳性神经元（1：1），表明该区域对DA输出的GABA能抑制能力可能很高。
The ventral midbrain is the primary source of dopamine- (DA) expressing neurons in most species. GABA-ergic and glutamatergic cell populations are intermixed among DA-expressing cells and purported to regulate both local and long-range dopamine neuron activity. Most work has been conducted in rodent models, however due to evolutionary expansion of the ventral midbrain in primates, the increased size and complexity of DA subpopulations warrants further investigation. Here, we quantified the number of DA neurons, and their GABA-ergic complement in classic DA cell groups A10 (midline ventral tegmental area nuclei [VTA] and parabrachial pigmented nucleus [PBP]), A9 (substantia nigra, pars compacta [SNc]) and A8 (retrorubral field [RRF]) in the macaque. Because the PBP is a disproportionately expanded feature of the A10 group, and has unique connectional features in monkeys, we analyzed A10 data by dividing it into ‘classic’ midline nuclei and the PBP. Unbiased stereology revealed total putative DA neuron counts to be 210,238 +/− 17,127 (A10 = 110,319 +/− 9,649, A9= 87,399 +/−7,751 and A8=12,520 +/− 827). Putative GABAergic neurons were fewer overall, and evenly dispersed across the DA subpopulations (GAD67= 71,215 +/− 5,663; A10=16,836 +/− 2,743; A9=24,855 +/− 3,144 and A8=12,633 +/− 3,557). Calculating the GAD67/TH ratio for each subregion revealed differential balances of these two cell types across the DA subregions. The A8 subregion had the highest complement of GAD67-positive neurons compared to TH-positive neurons (1:1), suggesting a potentially high capacity for GABAergic inhibition of DA output in this region.
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