Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue.
Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue.
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DOI：
10.1016/j.ejps.2013.11.005
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发表时间：
2014-06-16
影响因子：
4.6
通讯作者：
Stenken, Julie A.
中科院分区：
文献类型：
--
作者：
Keeler, Geoffrey D.;Durdik, Jeannine M.;Stenken, Julie A.
微透析采样是一种常用的技术，用于从实验室动物和人类组织的细胞外空间收集溶质。可以使用高分子量截留（MWCO）膜（100 kDa或更大）收集大分子量溶质。高MWCO膜需要向灌注液中添加高分子量葡聚糖或白蛋白，以防止通过超滤造成的液体损失。虽然这些灌注液添加剂常用于微透析采样过程中，但对这些化合物跨膜损失的组织反应知之甚少。在大鼠中比较了7天内对植入的含有不同微透析灌注液的微透析取样探针的组织反应。基础灌注液为补充有牛血清白蛋白（BSA）、大鼠血清白蛋白（RSA）、右旋糖酐-70或右旋糖酐-500的林格氏溶液。观察到对右旋糖酐-70的显著炎症反应。未观察到BSA和RSA之间的组织反应差异。在这些药物中，BSA、RSA和葡聚糖-500与葡聚糖-70相比产生显著降低的炎症反应。这项工作表明，在微透析采样灌注液中使用右旋糖酐-70应该被淘汰，并取代右旋糖酐-500或其他替代品。
Microdialysis sampling is a commonly used technique for collecting solutes from the extracellular space of tissues in laboratory animals and humans. Large molecular weight solutes can be collected using high molecular weight cutoff (MWCO) membranes (100 kDa or greater). High MWCO membranes require addition of high molecular weight dextrans or albumin to the perfusion fluid to prevent fluid loss via ultrafiltration. While these perfusion fluid additives are commonly used during microdialysis sampling, the tissue response to the loss of these compounds across the membrane is poorly understood. Tissue reactions to implanted microdialysis sampling probes containing different microdialysis perfusion fluids were compared over a 7-day time period in rats. The base perfusion fluid was Ringer’s solution supplemented with either bovine serum albumin (BSA), rat serum albumin (RSA), Dextran-70, or Dextran-500. A significant inflammatory response to Dextran-70 was observed. No differences in the tissue response between BSA and RSA were observed. Among these agents, the BSA, RSA, and Dextran-500 produced a significantly reduced inflammatory response compared to the Dextran-70. This work demonstrates that use of Dextran-70 in microdialysis sampling perfusion fluids should be eliminated and replaced with Dextran-500 or other alternatives.
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