Cryptic genetic diversity within the Anopheles nili group of malaria vectors in the equatorial forest area of Cameroon (Central Africa).
Cryptic genetic diversity within the Anopheles nili group of malaria vectors in the equatorial forest area of Cameroon (Central Africa).
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DOI：
10.1371/journal.pone.0058862
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Antonio-Nkondjio C
中科院分区：
文献类型：
--
作者：
Ndo C;Simard F;Kengne P;Awono-Ambene P;Morlais I;Sharakhov I;Fontenille D;Antonio-Nkondjio C
尼氏按蚊是撒哈拉以南非洲赤道森林和潮湿萨凡纳地区人类疟疾的重要传播媒介。然而，它在很大程度上仍然没有得到充分的研究，关于其种群生物学和遗传结构的数据非常缺乏。在这里，我们使用了核（即微卫星和核糖体DNA）和线粒体DNA标记的组合，探索和比较种群和物种之间的遗传多态性和分歧的水平在萨凡纳和森林地区的喀麦隆，中非。所有标记物均支持An内的当前分类。尼利集团然而，他们揭示了高遗传异质性内安。尼利公司深赤道森林环境。核标记显示该物种由5个高度分化的遗传谱系组成，其内部转录间隔区2（ITS2）序列的差异为1.8%至12.9%，这意味着近似的分歧时间为0.82至5.86百万年。然而，线粒体数据只检测到三个主要的细分，表明不同的进化历史的标记。这项研究揭示了An中额外的神秘遗传多样性。尼利公司在喀麦隆南部的深赤道森林环境中，这反映了这一环境中疟疾主要媒介的复杂人口历史。这些初步结果应得到进一步研究的补充，进一步研究将揭示这一物种群体在非洲雨林中的分布、流行病学重要性和演变历史，为深入比较研究非洲主要疟疾病媒的地方适应和物种形成提供机会。
The Anopheles nili group of mosquitoes includes important vectors of human malaria in equatorial forest and humid savannah regions of sub-Saharan Africa. However, it remains largely understudied, and data on its populations’ bionomics and genetic structure are crucially lacking. Here, we used a combination of nuclear (i.e. microsatellite and ribosomal DNA) and mitochondrial DNA markers to explore and compare the level of genetic polymorphism and divergence among populations and species of the group in the savannah and forested areas of Cameroon, Central Africa. All the markers provided support for the current classification within the An. nili group. However, they revealed high genetic heterogeneity within An. nili s.s. in deep equatorial forest environment. Nuclear markers showed the species to be composed of five highly divergent genetic lineages that differed by 1.8 to 12.9% of their Internal Transcribed Spacer 2 (ITS2) sequences, implying approximate divergence time of 0.82 to 5.86 million years. However, mitochondrial data only detected three major subdivisions, suggesting different evolutionary histories of the markers. This study enlightened additional cryptic genetic diversity within An. nili s.s. in the deep equatorial forest environment of South Cameroon, reflecting a complex demographic history for this major vector of malaria in this environment. These preliminary results should be complemented by further studies which will shed light on the distribution, epidemiological importance and evolutionary history of this species group in the African rainforest, providing opportunities for in-depth comparative studies of local adaptation and speciation in major African malaria vectors.
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非洲赤道森林地区的疟疾载体围绕菌群的种群结构。
影响因子：
3
作者：
Antonio-Nkondjio, Christophe;Ndo, Cyrille;Kengne, Pierre;Mukwaya, Louis;Awono-Ambene, Parfait;Fontenille, Didier;Simard, Frederic
通讯作者：
Simard, Frederic
DOI：
10.4269/ajtmh.2003.69.200
发表时间：
2003-08-01
影响因子：
3.3
作者：
Cohuet, A;Simard, F;Fontenille, D
通讯作者：
Fontenille, D
影响因子：
2.1
作者：
Antonio-Nkondjio, C;Awono-Ambene, P;Fontenille, D
通讯作者：
Fontenille, D
DOI：
10.1046/j.1471-8286.2003.00462.x
发表时间：
2003-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Berthomieu, A;Kengne, P;Weill, M
通讯作者：
Weill, M
影响因子：
1.9
作者：
CARNEVALE, P;LEGOFF, G;ROBERT, V
通讯作者：
ROBERT, V