Durability of three types of dual active ingredient long-lasting insecticidal net compared to a pyrethroid-only LLIN in Tanzania: methodology for a prospective cohort study nested in a cluster randomized controlled trial.
Durability of three types of dual active ingredient long-lasting insecticidal net compared to a pyrethroid-only LLIN in Tanzania: methodology for a prospective cohort study nested in a cluster randomized controlled trial.
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DOI：
10.1186/s12936-022-04119-4
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发表时间：
2022-03-19
期刊：
影响因子：
3
通讯作者：
Rowland M
中科院分区：
文献类型：
--
作者：
Martin JL;Messenger LA;Mosha FW;Lukole E;Mosha JF;Kulkarni M;Churcher TS;Sherrard-Smith E;Manjurano A;Protopopoff N;Rowland M
由于病媒种群对拟除虫菊酯的抗药性普遍存在选择性，长效驱虫蚊帐在防治疟疾方面取得的进展受到威胁。迫切需要使用非拟除虫菊酯类杀虫剂的长效驱虫蚊帐。本研究旨在评估三类双活性成分（A.I.）长效驱虫蚊帐使用源自世卫组织长效驱虫蚊帐既定检测方法的技术，以制定新的评价标准。将在坦桑尼亚Misungwi区的40个集群中进行一项世卫组织III期活性成分和纺织品耐久性研究。将对以下处理进行评价：（1）Interceptor®G2，结合溴虫腈和拟除虫菊酯-氯氰菊酯，（2）用吡丙醚和氯氰菊酯处理的皇家卫士®，（3）Olyset™ Plus，其中包含增效醚和拟除虫菊酯-氯氰菊酯，以及（4）参比标准品-氯氰菊酯LLIN（Interceptor®）。在分发后6个月、12个月、24个月和36个月，每个干预组将在5个集群中对750个蚊帐进行随访，以评估存活率和孔指数。第二批每种类型的1 950顶蚊帐将分成10个组群，其中30顶长效驱虫蚊帐将每6个月取出进行生物功效和化学分析，最长36个月，另外30顶蚊帐将每年收集进行实验性棚屋试验。将使用锥体生物测定和隧道试验对冈比亚按蚊的敏感和耐药实验室菌株进行生物效力评估。将在六个实验小屋中比较实地收集的蚊帐的功效。主要结果将是接触后72小时内的按蚊死亡率、吸血和使用卵巢解剖的卵子成熟，以评估对生殖力的影响。研究结果将有助于为合作伙伴A.I.制定生物功效和物理耐久性标准，与cRCT流行病学和昆虫学结果的关系，并完善每类长效杀虫剂的首选产品特征。如适用，将生物测定和hut结果拟合至传播模型，以估计与cRCT结果的相关性。试验注册号：NCT 03554616。在线版本包含补充材料，可通过10.1186/s12936-022-04119-4获得。
Progress achieved by long-lasting insecticidal nets (LLINs) against malaria is threatened by widespread selection of pyrethroid resistance among vector populations. LLINs with non-pyrethroid insecticides are urgently needed. This study aims to assess the insecticide and textile durability of three classes of dual-active ingredient (A.I.) LLINs using techniques derived from established WHO LLIN testing methods to set new standards of evaluation. A WHO Phase 3 active ingredients and textile durability study will be carried out within a cluster randomized controlled trial in 40 clusters in Misungwi district, Tanzania. The following treatments will be evaluated: (1) Interceptor®G2 combining chlorfenapyr and the pyrethroid alpha-cypermethrin, (2) Royal Guard® treated with pyriproxyfen and alpha-cypermethrin, (3) Olyset™ Plus which incorporates a synergist piperonyl butoxide and the pyrethroid permethrin, and (4) a reference standard alpha-cypermethrin only LLIN (Interceptor®). 750 nets will be followed in 5 clusters per intervention arm at 6, 12, 24 and 36 months post distribution for survivorship and hole index assessment. A second cohort of 1950 nets per net type will be identified in 10 clusters, of which 30 LLINs will be withdrawn for bio-efficacy and chemical analysis every 6 months up to 36 months and another 30 collected for experimental hut trials every year. Bio-efficacy will be assessed using cone bioassays and tunnel tests against susceptible and resistant laboratory strains of Anopheles gambiae sensu stricto. Efficacy of field-collected nets will be compared in six experimental huts. The main outcomes will be Anopheles mortality up to 72 h post exposure, blood feeding and egg maturation using ovary dissection to assess impact on fecundity. Study findings will help develop bio-efficacy and physical durability criteria for partner A.I., in relation to the cRCT epidemiological and entomological outcomes, and refine preferred product characteristics of each class of LLIN. If suitable, the bioassay and hut outcomes will be fitted to transmission models to estimate correlation with cRCT outcomes. Trial registration number: NCT03554616. The online version contains supplementary material available at 10.1186/s12936-022-04119-4.
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影响因子：
11.8
作者：
Asidi A;N'Guessan R;Akogbeto M;Curtis C;Rowland M
通讯作者：
Rowland M
影响因子：
2.9
作者：
Mosha JF;Kulkarni MA;Messenger LA;Rowland M;Matowo N;Pitt C;Lukole E;Taljaard M;Thickstun C;Manjurano A;Mosha FW;Kleinschmidt I;Protopopoff N
通讯作者：
Protopopoff N
影响因子：
3.7
作者：
Pennetier C;Bouraima A;Chandre F;Piameu M;Etang J;Rossignol M;Sidick I;Zogo B;Lacroix MN;Yadav R;Pigeon O;Corbel V
通讯作者：
Corbel V
影响因子：
4.6
作者：
Matowo NS;Martin J;Kulkarni MA;Mosha JF;Lukole E;Isaya G;Shirima B;Kaaya R;Moyes C;Hancock PA;Rowland M;Manjurano A;Mosha FW;Protopopoff N;Messenger LA
通讯作者：
Messenger LA
影响因子：
3
作者：
Owusu, Henry F.;Muller, Pie
通讯作者：
Muller, Pie