Measurement of glutathione in normal volunteers and stroke patients at 3T using J-difference spectroscopy with minimized subtraction errors.
Measurement of glutathione in normal volunteers and stroke patients at 3T using J-difference spectroscopy with minimized subtraction errors.
复制标题
DOI：
10.1002/jmri.21832
复制
发表时间：
2009-08
影响因子：
4.4
通讯作者：
Warach, Steven
中科院分区：
文献类型：
--
作者：
An, Li;Zhang, Yan;Thomasson, David M.;Latour, Lawrence L.;Baker, Eva H.;Shen, Jun;Warach, Steven
建立并优化一种测定脑谷胱甘肽（GSH）水平的1H MRS方法。通过模拟实验和密度算子模拟来确定使用j差光谱编辑技术测量3t下GSH的最佳TE。采用一种新的光谱对齐方法（自适应光谱配准）对11名正常志愿者（43次测量）和5名中风患者（10次测量）的体内数据进行处理。在模体实验和忽略松弛效应的密度算子模拟中，在TE≈131 ms时获得了接近最大GSH信号（2.95 ppm），且n -乙酰-天冬氨酸（NAA）信号干扰最小。采用自适应光谱配准，健康志愿者的谷胱甘肽水平为1.20±0.14 mM （mean±SD）。脑卒中患者的GSH水平病变区为1.19±0.24 mM，对侧正常组织为1.25±0.19 mM。相比之下，仅使用NAA单线态（2.01 ppm）作为导航器进行光谱对准时，标准偏差明显更大。使用j差进行谱编辑是测量志愿者和中风患者谷胱甘肽水平的可靠方法。
To develop and optimize a 1H MRS method for measuring brain glutathione (GSH) levels. Phantom experiments and density operator simulations were performed to determine the optimal TE for measuring GSH at 3 T using J-difference spectral editing. In vivo data collected from eleven normal volunteers (forty-three measurements) and five stroke patients (ten measurements) were processed using a new spectral alignment method (adaptive spectral registration). In phantom experiments and density operator simulations where relaxation effects were ignored, close to maximum GSH signal (2.95 ppm) was obtained at TE ≈ 131 ms with minimum N-acetyle-aspartate (NAA) signal interference. Using adaptive spectral registration, GSH levels in healthy volunteers were found to be 1.20 ± 0.14 mM (mean ± SD). GSH levels in stroke patients were found to be 1.19 ± 0.24 mM in lesion and 1.25 ± 0.19 mM in contralateral normal tissue. In comparison, the standard deviations were significantly larger when only the NAA singlet (2.01 ppm) was used as a navigator for spectral alignment. Spectral editing using J-differences is a reliable method for measuring GSH levels in volunteers and stroke patients.
登录
查看更多内容
影响因子：
3.3
作者：
Kreis, R;Hofmann, L;Hüppi, PS
通讯作者：
Hüppi, PS
影响因子：
8.3
作者：
Shimizu, H;Kiyohara, Y;Iida, M
通讯作者：
Iida, M
影响因子：
3.3
作者：
KREIS, R;ERNST, T;ROSS, BD
通讯作者：
ROSS, BD
影响因子：
3.3
作者：
Choi, IY;Lee, SP;Shen, J
通讯作者：
Shen, J
DOI：
10.1006/jmra.1994.1008
发表时间：
1994-01-01
期刊：
JOURNAL OF MAGNETIC RESONANCE SERIES A
影响因子：
--
作者：
SMITH, SA;LEVANTE, TO;ERNST, RR
通讯作者：
ERNST, RR