Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice.
Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice.
复制标题
下丘脑外侧区谷氨酸能神经元及其对外侧缰核的投射调节小鼠异氟烷的麻醉效力
DOI：
10.1007/s12264-021-00674-z
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发表时间：
2021-07
影响因子：
5.6
通讯作者：
Dong H
中科院分区：
文献类型：
--
作者：
Zhao S;Li R;Li H;Wang S;Zhang X;Wang D;Guo J;Li H;Li A;Tong T;Zhong H;Yang Q;Dong H
下丘脑外侧区（LHA）在调节意识转换中起着关键作用，其中包括食欲能神经元、gaba能神经元和黑色素浓缩激素神经元。谷氨酸能神经元在LHA中有大量分布，但其与麻醉相关的作用尚未被探索。本研究发现，基因消融LHA谷氨酸能神经元可缩短小鼠异氟醚麻醉诱导时间，延长小鼠异氟醚麻醉恢复时间。相比之下，LHA谷氨酸能神经元的化学发生激活增加了麻醉时间，减少了恢复时间。在麻醉维持期间，LHA谷氨酸能神经元的光遗传激活减少了脑电图的爆发抑制模式，并将脑电图特征转移到唤醒模式。光刺激LHA向外侧链（LHb）的谷氨酸能投射也促进了麻醉的出现和麻醉深度向较浅的过渡。总的来说，LHA谷氨酸能神经元及其对LHb的投射调节麻醉效力和脑电图特征。
The lateral hypothalamic area (LHA) plays a pivotal role in regulating consciousness transition, in which orexinergic neurons, GABAergic neurons, and melanin-concentrating hormone neurons are involved. Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has not been explored. Here, we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice. In contrast, chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery. Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram (EEG) and shifted EEG features to an arousal pattern. Photostimulation of LHA glutamatergic projections to the lateral habenula (LHb) also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level. Collectively, LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features.
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影响因子：
16.2
作者：
Li, Y;Gao, XB;van den Pol, AN
通讯作者：
van den Pol, AN
DOI：
10.1016/j.cub.2018.05.025
发表时间：
2018-07-09
期刊：
Current biology : CB
影响因子：
--
作者：
Pal D;Dean JG;Liu T;Li D;Watson CJ;Hudetz AG;Mashour GA
通讯作者：
Mashour GA
影响因子：
5.3
作者：
Lee, MG;Hassani, OK;Jones, BE
通讯作者：
Jones, BE
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
64.8
作者：
Adamantidis, Antoine R.;Zhang, Feng;De Lecea, Luis
通讯作者：
De Lecea, Luis