A potential role for B cells in suppressed immune responses in cord blood transplant recipients.
A potential role for B cells in suppressed immune responses in cord blood transplant recipients.
复制标题
B细胞在绳索血液移植受体中抑制免疫反应中的潜在作用。
DOI：
10.1038/bmt.2012.104
复制
发表时间：
2013-01
影响因子：
4.8
通讯作者：
Stiff PJ
中科院分区：
文献类型：
--
作者：
Beaudette-Zlatanova BC;Le PT;Knight KL;Zhang S;Zakrzewski S;Parthasarathy M;Stiff PJ
我们评估了 58 名成年人的免疫重建情况，这些人在移植后一年内每隔 90 天接受同种异体兄弟姐妹 (allosib)、匹配的无关供体 (MUD) 或脐带血 (CB) 的造血干细胞移植。与 allosib 和 MUD 接受者相比，CB 接受者在前 100 天内的感染发生率更高。与 MUD 接受者相比，CB 接受者在 90 天时的循环 T 细胞数量较低，在 180 天时与 allosib 接受者相比。对患者淋巴细胞 TCR Vβ 互补决定区 3 (CDR3) 的谱型分析显示，几乎所有患者的 TCR 库在 360 天时仍然缺乏多样化。相比之下，与 allosib 受者相比，CB 受者在移植后第一年以及 9-12 个月时的循环 B 细胞数量显着高于 MUD 受者。 B 细胞受体 VH CDR3 的光谱类型分析表明，大多数患者的 B 细胞库在 90 天时发生了多样化。来自所检测的 CB 受体的 CD5pos B 细胞在移植后早期表达细胞内 IL-10。我们的数据表明，除了 T 细胞之外，B 细胞可能在 CB 移植患者免疫反应受损中发挥作用。
We evaluated immune reconstitution in 58 adults who received hematopoietic stem cell transplants from allogeneic siblings (allosib), matched unrelated donors (MUD), or cord blood (CB) at 90-day intervals for one year post-transplant. CB recipients had a higher incidence of infections in the first 100 days compared to allosib and MUD recipients. The number of circulating T cells was lower in CB recipients compared to MUD recipients at 90 days and compared to allosib recipients at 180 days. Spectratype analysis of the TCR Vβ complementarity determining region 3 (CDR3) of patient lymphocytes revealed that the TCR repertoire remained poorly diversified even at 360 days in nearly all patients. In contrast, the number of circulating B cells was significantly elevated in CB recipients compared to allosib recipients throughout the first year post-transplant and compared to MUD recipients at 9-12 months. Spectratype analysis of the B cell receptor VH CDR3 showed that the B cell repertoire was diversified in most patients by 90 days. CD5pos B cells from assayed CB recipients expressed intracellular IL-10 early post-transplant. Our data suggest that B cells, in addition to T cells, may play a role in impaired immune responses in CB transplant patients.
登录
查看更多内容
影响因子：
32.4
作者：
Mizoguchi, A;Mizoguchi, E;Bhan, AK
通讯作者：
Bhan, AK
影响因子：
20.3
作者：
Rocha, V;Cornish, J;Gluckman, E
通讯作者：
Gluckman, E
影响因子：
8.6
作者：
Marie-Cardine, Aude;Divay, Florence;Jacquot, Serge
通讯作者：
Jacquot, Serge
影响因子：
4.3
作者：
Jacobson, Caron A.;Turki, Amin T.;Ritz, Jerome
通讯作者：
Ritz, Jerome
影响因子：
158.5
作者：
Laughlin, MJ;Eapen, M;Horowitz, MM
通讯作者：
Horowitz, MM