The receptor tyrosine kinase EphB4 and ephrin-B ligands restrict angiogenic growth of embryonic veins in Xenopus laevis.
The receptor tyrosine kinase EphB4 and ephrin-B ligands restrict angiogenic growth of embryonic veins in Xenopus laevis.
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受体酪氨酸激酶 EphB4 和肝配蛋白 B 配体限制非洲爪蟾胚胎静脉的血管生成生长。
作者：
P. Helbling;Didier M.E. Saulnier;A. Brändli
引导组织和器官中胚胎血管形成的线索和信号系统尚不清楚。Eph受体酪氨酸激酶家族成员及其细胞膜锚定配体ephrin在胚胎发生过程中控制细胞迁移，特别是轴突引导和神经嵴迁移中发挥了重要作用。本文研究了EphB受体及其配体在非洲爪蟾胚胎血管发育中的作用。在对蝌蚪期非洲爪蟾胚胎EphB4受体表达情况的调查中，我们检测到了EphB4受体在后主静脉及其衍生物——体间静脉中的表达。其他EphB受体，包括EphB1、EphB2和EphB3，在非洲爪蟾的血管中均未见表达，这表明EphB4是非洲爪蟾早期胚胎脉管系统的主要EphB受体。此外，我们发现ephrin-B配体在血管生成生长过程中，与EphB4互补表达在相邻的体质体中。我们通过RNA注射实验研究EphB4及其配体在体间静脉发育中的作用。在非洲爪蟾胚胎中，EphB4显性阴性受体或ephrin-B配体的错误表达破坏EphB4信号通路，可导致体间静脉向相邻体间组织异常生长。我们的研究结果表明，EphB4和b类ephrin可能通过介导内皮细胞迁移的排斥引导信号来调节血管生成。
The cues and signaling systems that guide the formation of embryonic blood vessels in tissues and organs are poorly understood. Members of the Eph family of receptor tyrosine kinases and their cell membrane-anchored ligands, the ephrins, have been assigned important roles in the control of cell migration during embryogenesis, particularly in axon guidance and neural crest migration. Here we investigated the role of EphB receptors and their ligands during embryonic blood vessel development in Xenopus laevis. In a survey of tadpole-stage Xenopus embryos for EphB receptor expression, we detected expression of EphB4 receptors in the posterior cardinal veins and their derivatives, the intersomitic veins. Vascular expression of other EphB receptors, including EphB1, EphB2 or EphB3, could however not be observed, suggesting that EphB4 is the principal EphB receptor of the early embryonic vasculature of Xenopus. Furthermore, we found that ephrin-B ligands are expressed complementary to EphB4 in the somites adjacent to the migratory pathways taken by intersomitic veins during angiogenic growth. We performed RNA injection experiments to study the function of EphB4 and its ligands in intersomitic vein development. Disruption of EphB4 signaling by dominant negative EphB4 receptors or misexpression of ephrin-B ligands in Xenopus embryos resulted in intersomitic veins growing abnormally into the adjacent somitic tissue. Our findings demonstrate that EphB4 and B-class ephrins act as regulators of angiogenesis possibly by mediating repulsive guidance cues to migrating endothelial cells.
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DOI：
--
发表时间：
1996-12
期刊：
Kidney international. Supplement
影响因子：
--
作者：
Thomas O. Daniel;E. Stein;D. Cerretti;St John Pl;B. Robert;Dale R. Abrahamson
通讯作者：
Thomas O. Daniel;E. Stein;D. Cerretti;St John Pl;B. Robert;Dale R. Abrahamson
DOI：
10.1164/ajrccm/140.4.1097
发表时间：
1989-10-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
NODEN, DM
通讯作者：
NODEN, DM
影响因子：
56.9
作者：
Suri, C;McClain, J;Yancopoulos, GD
通讯作者：
Yancopoulos, GD
影响因子：
56.9
作者：
PANDEY, A;SHAO, HN;DIXIT, VM
通讯作者：
DIXIT, VM
影响因子：
2.7
作者：
COFFIN, JD;HARRISON, J;HEIMARK, R
通讯作者：
HEIMARK, R