Effect of FTO Expression and Polymorphism on Fat Deposition in Suzhong Pigs.
Effect of FTO Expression and Polymorphism on Fat Deposition in Suzhong Pigs.
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DOI：
10.5713/ajas.2013.13055
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Ren S
中科院分区：
文献类型：
--
作者：
Fu Y;Li L;Ren S
脂肪量和肥胖相关基因（FTO）在人和小鼠的食欲控制和能量消耗中起重要作用。为探讨FTO基因表达对苏忠猪脂肪沉积的影响，采用RT-PCR方法检测了苏忠猪16个组织中FTO基因mRNA的表达量，采用Western blot方法检测了苏忠猪5个组织中FTO蛋白的表达量，并分析了FTO基因多态性与肉质性状的相关性。RT-PCR结果显示，FTO mRNA在16个组织中均有表达，差异显著（p<0.05），背膘中的表达量显著高于其他组织（p<0.05），背最长肌中的表达量次之（p<0.05）。Western blot结果表明，FTO蛋白在背膘和背最长肌中有高表达。高脂猪各组织中FTO mRNA和蛋白的表达量显著高于低脂猪（p<0.05），表明FTO的表达对脂肪沉积有促进作用。FTO基因多态性分析结果表明，A227 G位点G等位基因对脂肪沉积有显著的促进作用，可能是改良苏忠猪肉质的重要候选基因。
Fat mass and obesity associated gene (FTO) plays an important role in appetite control and energy consumption in human and mice. In order to examine FTO expression influence on fat deposition in Suzhong pigs, FTO mRNA expression was detected in 16 tissues by RT-PCR, FTO protein expression was detected in 5 tissues by western blot, and association of FTO polymorphism with meat quality traits was analyzed in Suzhong populations with 714 records. RT-PCR results revealed that FTO mRNA was expressed in all sixteen tissues with significant differences (p<0.05), expression in backfat was significantly higher than that of any other tissue (p<0.05), and expression in longissimus dorsi muscle had the second highest significance level (p<0.05). Western blot results demonstrated that FTO protein was highly expressed in backfat and longissimus dorsi muscle. Furthermore, FTO mRNA and protein expression in tissues of high-fat pigs was significantly higher than that of low-fat pigs (p<0.05), suggesting FTO expression had advantageous effects on fat deposition. FTO polymorphism results evidenced that at A227G locus, G allele seemed to have advantageous effects on fat deposition, indicating it could be a significant candidate gene for improving pork quality in Suzhong pigs.
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