Exploiting pathogens' tricks of the trade for engineering of plant disease resistance: challenges and opportunities.

Exploiting pathogens' tricks of the trade for engineering of plant disease resistance: challenges and opportunities.
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DOI:
10.1111/1751-7915.12017
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发表时间:
2013-05
影响因子:
5.7
通讯作者:
Manners JM
Manners JM
中科院分区:
工程技术2区
文献类型:
--
作者:
Grant MR;Kazan K;Manners JM

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随着我们对病原体效应策略及其宿主靶标多样性的理解的扩展,越来越明显的是,需要部署工程化广谱抗性的新方法。植物-微生物相互作用的高时间基因表达数据的日益增加的可用性使得能够明智地选择启动子以在特定胁迫条件下微调表达的时间和幅度。因此,我们可以考虑设计一系列转基因品系,以干扰病原体毒力策略,靶向植物激素信号传导或部署特定的抗病基因。这种方法的一个优点是激素信号是通用的,所以如果这种策略是有效的,它可以很容易地在一系列作物物种中实施。此外,多条重新布线的线路可以交叉,以产生对病原体更有效的反应。
With expansion of our understanding of pathogen effector strategies and the multiplicity of their host targets, it is becoming evident that novel approaches to engineering broad-spectrum resistance need to be deployed. The increasing availability of high temporal gene expression data of a range of plant–microbe interactions enables the judicious choices of promoters to fine-tune timing and magnitude of expression under specified stress conditions. We can therefore contemplate engineering a range of transgenic lines designed to interfere with pathogen virulence strategies that target plant hormone signalling or deploy specific disease resistance genes. An advantage of such an approach is that hormonal signalling is generic so if this strategy is effective, it can be easily implemented in a range of crop species. Additionally, multiple re-wired lines can be crossed to develop more effective responses to pathogens.
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