Postnatal binge-like alcohol exposure decreases dendritic complexity while increasing the density of mature spines in mPFC Layer II/III pyramidal neurons.
Postnatal binge-like alcohol exposure decreases dendritic complexity while increasing the density of mature spines in mPFC Layer II/III pyramidal neurons.
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DOI：
10.1002/syn.20711
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发表时间：
2010-02
期刊：
影响因子：
2.3
通讯作者：
Klintsova, Anna Y.
中科院分区：
文献类型：
--
作者：
Hamilton, Gillian F.;Whitcher, Lee T.;Klintsova, Anna Y.
人类产前暴露于酒精会导致广泛的缺陷，统称为胎儿酒精谱系障碍。在这些缺陷中，认知障碍是最具破坏性和持久性的。具体来说，执行功能的认知障碍表明前额叶皮层（PFC）受损。一些外部刺激，如吗啡，慢性应激和母体应激已被发现改变PFC内的树突状细胞的结构。在这项研究中，使用三组大鼠幼崽：插管与酒精（5.25克/公斤/天; AE），假插管（SI），或PD 4 -9的酒精对照（SC）。在PD 26 -30，将大鼠麻醉，用盐水灌注，并处理脑用于Golgi-Cox染色。基底树突的复杂性，棘密度和棘表型进行了评价，第II/III层神经元在内侧PFC。结果表明，AE大鼠有一个改变基底树突树的复杂性，由于在接近神经元索马的交叉点的长度和数量显着减少。此外，基底树突的棘密度模式保持不变，而成熟与未成熟棘的密度显着变化。这些效应在顶端树突中没有观察到，表明酒精对单个细胞中不同神经元部分的影响。此外，这些结果表明，丘脑投射对索马和基底树突的支配可能起作用。因此，我们的数据表明，出生后暴露于酒精产生的大鼠青春期PFC的神经元组织的变化，可能会影响前额叶依赖的行为任务的性能。
Prenatal exposure to alcohol in humans can result in a wide range of deficits collectively referred to as Fetal Alcohol Spectrum Disorders. Of these deficits, cognitive impairments are among the most debilitating and long-lasting. Specifically, cognitive impairments in executive functioning suggest damage to the prefrontal cortex (PFC). Several external stimuli, such as morphine, chronic stress and maternal stress have been found to alter the dendritic structure of cells within the PFC. In this study, three groups of rat pups were used: intubated with alcohol (5.25g/kg/day; AE), sham intubated (SI), or suckle controls (SC) on PD4-9. On PD26-30 rats were anesthetized, perfused with saline and brains were processed for Golgi-Cox staining. Basilar dendrite complexity, spine density and spine phenotypes were evaluated for Layer II/III neurons in the medial PFC. Results indicate that AE rats have an altered basilar dendritic tree complexity due to a significant decrease in both length and number of intersections in proximity to the neuronal soma. Furthermore, spine density patterns of basilar dendrites remain unchanged while the density of mature versus immature spines significantly changes. These effects were not seen in the apical dendrites, indicating alcohol’s influence on different neuronal parts in a single cell. In addition, these results suggest that the innervations of the soma and basilar dendrites by thalamic projections may play a role. Thus, our data demonstrates that postnatal exposure to alcohol produces changes in the neuronal organization of rat adolescent PFC that may affect the performance on prefrontal-dependant behavioral tasks.
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影响因子：
11
作者：
HARPER, C;CORBETT, D
通讯作者：
CORBETT, D
影响因子：
2.5
作者：
KALSBEEK, A;VOORN, P;UYLINGS, HBM
通讯作者：
UYLINGS, HBM
影响因子：
3.8
作者：
Granato, A;Di Rocco, F;Giannetti, S
通讯作者：
Giannetti, S
影响因子：
1.4
作者：
FERRER, I;GALOFRE, E
通讯作者：
GALOFRE, E
DOI：
10.1073/pnas.0807041106
发表时间：
2009-01-20
影响因子：
11.1
作者：
Liston, C.;McEwen, B. S.;Casey, B. J.
通讯作者：
Casey, B. J.