Elevated extracellular calcium concentrations induce type X collagen synthesis in chondrocyte cultures.
Elevated extracellular calcium concentrations induce type X collagen synthesis in chondrocyte cultures.
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DOI：
10.1083/jcb.115.4.1171
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发表时间：
1991-11
期刊：
影响因子：
--
通讯作者：
Schmid TM
中科院分区：
文献类型：
--
作者：
Bonen DK;Schmid TM
从12 d鸡胫跗骨段（未成熟软骨细胞）和2区和3区（成熟软骨细胞）中分离出不同分化阶段的软骨细胞。两种来源的软骨细胞在细胞形态、生长速度和X型胶原蛋白的产生方面存在差异。在24 h内，2区和3区软骨细胞比跗骨软骨细胞多合成800倍的X型胶原。研究外源性CaCl2（5和10 mM）对成熟和未成熟软骨细胞合成X型胶原的影响。2区和3区软骨细胞与CaCl2型X胶原蛋白孵育72小时后，增加了8倍的5毫米和10倍的10毫米Ca2+。[3H]添加5和10 mM CaCl2时，脯氨酸在培养基和基质大分子中的掺入量分别增加11%和32%。在这72小时内，II型胶原合成不受细胞外Ca2+升高的影响。对跗骨软骨细胞的类似研究表明，在48小时内，与5和10 mM Ca2+对照相比，X型胶原水平分别达到4倍和15倍的时间和剂量依赖性CaCl2反应。升高的细胞外Ca2+对细胞增殖没有影响。这些观察结果提供了细胞外Ca2+升高诱导X型胶原合成的第一个直接证据。
Chondrocytes at different stages of cellular differentiation were isolated from the tarsal element (immature chondrocytes) and zones 2 and 3 (mature chondrocytes) of 12-d chick embryo tibiotarsus. The chondrocytes from the two sources differed in their cell morphologies, growth rate and production of type X collagen. In 24 h, zone 2 and 3 chondrocytes synthesized 800 times more type X collagen than tarsal chondrocytes. The effect of exogenous CaCl2 (5 and 10 mM) on the synthesis of type X collagen by both mature and immature chondrocytes was tested. After a 72-h incubation of zone 2 and 3 chondrocytes with CaCl2 type X collagen increased 8-fold with 5 mM and 10-fold with 10 mM Ca2+. [3H]Proline incorporation into culture medium and matrix macromolecules increased 11 and 32% with 5 and 10 mM CaCl2, respectively. Type II collagen synthesis was not affected by elevated extracellular Ca2+ during this 72-h period. Similar studies with tarsal chondrocytes demonstrated a time- and dose-dependent response to CaCl2 with type X collagen levels reaching a 4-fold and 15-fold increase over controls with 5 and 10 mM Ca2+, respectively, at 48 h. Elevated extracellular Ca2+ had no effect on cell proliferation. These observations offer the first direct evidence of the induction of type X collagen synthesis with elevated extracellular Ca2+.
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影响因子：
2.9
作者：
LABARCA, C;PAIGEN, K
通讯作者：
PAIGEN, K
DOI：
10.2106/00004623-197860050-00007
发表时间：
1978-01-01
影响因子：
5.3
作者：
BRIGHTON, CT;HUNT, RM
通讯作者：
HUNT, RM
影响因子：
2.9
作者：
DOWNS, TR;WILFINGER, WW
通讯作者：
WILFINGER, WW
X型胶原蛋白表达的比较研究在正常和rachitic鸡外骨软骨中。
影响因子：
7.8
作者：
Kwan, A P;Dickson, I R;Freemont, A J;Grant, M E
通讯作者：
Grant, M E
影响因子：
3.5
作者：
KIELTY, CM;HULMES, DJS;GRANT, ME
通讯作者：
GRANT, ME